TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Shkarina, Svetlana
A1  - Shkarin, Roman
A1  - Weinhardt, Venera
A1  - Melnik, Elizaveta
A1  - Vacun, Gabriele
A1  - Kluger, Petra
A1  - Loza, Kateryna
A1  - Epple, Matthias
T1  - 3D biodegradable scaffolds of polycaprolactone with silicate-containing hydroxyapatite microparticles for bone tissue engineering: high-resolution tomography and in vitro study
JF  - Scientific reports
N2  - To date, special interest has been paid to composite scaffolds based on polymers enriched with hydroxyapatite (HA). However, the role of HA containing different trace elements such as silicate in the structure of a polymer scaffold has not yet been fully explored. Here, we report the potential use of silicate-containing hydroxyapatite (SiHA) microparticles and microparticle aggregates in the predominant range from 2.23 to 12.40 μm in combination with polycaprolactone (PCL) as a hybrid scaffold with randomly oriented and well-aligned microfibers for regeneration of bone tissue. Chemical and mechanical properties of the developed 3D scaffolds were investigated with XRD, FTIR, EDX and tensile testing. Furthermore, the internal structure and surface morphology of the scaffolds were analyzed using synchrotron X-ray μCT and SEM. Upon culturing human mesenchymal stem cells (hMSC) on PCL-SiHA scaffolds, we found that both SiHA inclusion and microfiber orientation affected cell adhesion. The best hMSCs viability was revealed at 10 day for the PCL-SiHA scaffolds with well-aligned structure (~82%). It is expected that novel hybrid scaffolds of PCL will improve tissue ingrowth in vivo due to hydrophilic SiHA microparticles in combination with randomly oriented and well-aligned PCL microfibers, which mimic the structure of extracellular matrix of bone tissue.
Y1  - 2018
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-22355
SN  - 2045-2322
SS  - 2045-2322
U6  - https://doi.org/10.1038/s41598-018-27097-7
DO  - https://doi.org/10.1038/s41598-018-27097-7
VL  - 8
IS  - Aufsatz 8907
SP  - 1
EP  - 13
S1  - 13
PB  - Macmillan Publishers Limited
CY  - London
ER  -