TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Nellinger, Svenja
A1  - Schmidt, Isabelle
A1  - Heine, Simon
A1  - Volz, Ann-Cathrin
A1  - Kluger, Petra
T1  - Adipose stem cell-derived extracellular matrix represents a promising biomaterial by inducing spontaneous formation of prevascular-like structures by mvECs
JF  - Biotechnology & Bioengineering
N2  - Tissue constructs of physiologically relevant scale require a vascular system to maintain cell viability. However, in vitro vascularization of engineered tissues is still a major challenge. Successful approaches are based on a feeder layer (FL) to support vascularization. Here, we investigated whether the supporting effect on the self‐assembled formation of prevascular‐like structures by microvascular endothelial cells (mvECs) originates from the FL itself or from its extracellular matrix (ECM). Therefore, we compared the influence of ECM, either derived from adipose‐derived stem cells (ASCs) or adipogenically differentiated ASCs, with the classical cell‐based FL. All cell‐derived ECM (cdECM) substrates enabled mvEC growth with high viability. Prevascular‐like structures were visualized by immunofluorescence staining of endothelial surface protein CD31 and could be observed on all cdECM and FL substrates but not on control substrate collagen I. On adipogenically differentiated ECM, longer and higher branched structures could be found compared with stem cell cdECM. An increased concentration of proangiogenic factors was found in cdECM substrates and FL approaches compared with controls. Finally, the expression of proteins associated with tube formation (E‐selectin and thrombomodulin) was confirmed. These results highlight cdECM as promising biomaterial for adipose tissue engineering by inducing the spontaneous formation of prevascular‐like structures by mvECs.
KW  - adipose-derived stem cells
KW  - biomaterials
KW  - extracellular matrix
KW  - prevascular-like structures
KW  - tissue engineering
Y1  - 2020
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-28355
SN  - 0006-3592
SS  - 0006-3592
U6  - https://doi.org/10.1002/bit.27481
DO  - https://doi.org/10.1002/bit.27481
VL  - 117
IS  - 10
SP  - 3160
EP  - 3172
S1  - 13
PB  - Wiley
CY  - New York, NY
ER  -