TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Kluger, Petra
A1  - Nellinger, Svenja
A1  - Heine, Simon
A1  - Volz, Ann-Cathrin
T1  - Cell-derived extracellular matrix as maintaining biomaterial for adipogenic differentiation
JF  - Current Directions in Biomedical Engineering
N2  - The extracellular matrix (ECM) naturally surrounds cells in humans, and therefore represents the ideal biomaterial for tissue engineering. ECM from different tissues exhibit different composition and physical characteristics. Thus, ECM provides not only physical support but also contains crucial biochemical signals that influence cell adhesion, morphology, proliferation and differentiation. Next to native ECM from mature tissue, ECM can also be obtained from the in vitro culture of cells. In this study, we aimed to highlight the supporting effect of cell-derived- ECM (cdECM) on adipogenic differentiation. ASCs were seeded on top of cdECM from ASCs (scdECM) or pre-adipocytes (acdECM). The impact of ECM on cellular activity was determined by LDH assay, WST I assay and BrdU assay. A supporting effect of cdECM substrates on adipogenic differentiation was determined by oil red O staining and subsequent quantification. Results revealed no effect of cdECM substrates on cellular activity. Regarding adipogenic differentiation a supporting effect of cdECM substrates was obtained compared to control. With these results, we confirm cdECM as a promising biomaterial for adipose tissue engineering.
KW  - extracellular matrix
KW  - adipose-derived stem cells
KW  - differentiation
KW  - biomaterial
Y1  - 2020
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-29096
SN  - 2364-5504
SS  - 2364-5504
U6  - https://doi.org/10.1515/cdbme-2020-3106
DO  - https://doi.org/10.1515/cdbme-2020-3106
VL  - 6
IS  - 3
SP  - 1
EP  - 4
S1  - 4
PB  - De Gruyter
CY  - Berlin
ER  -