TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Daum, Ruben
A1  - Visser, Dmitri
A1  - Wild, Constanze
A1  - Kutuzova, Larysa
A1  - Schneider, Maria
A1  - Lorenz, Günter
A1  - Weiss, Martin
A1  - Hinderer, Svenja
A1  - Stock, Ulrich
A1  - Seifert, Martina
A1  - Schnke-Layland, Katja
T1  - Fibronectin adsorption on electrospun synthetic vascular grafts attracts endothelial progenitor cells and promotes endothelialization in dynamic in vitro culture
JF  - Cells : open access journal
N2  - Appropriate mechanical properties and fast endothelialization of synthetic grafts are key to ensure long-term functionality of implants. We used a newly developed biostable polyurethane elastomer (TPCU) to engineer electrospun vascular scaffolds with promising mechanical properties (E-modulus: 4.8 ± 0.6 MPa, burst pressure: 3326 ± 78 mmHg), which were biofunctionalized with fibronectin (FN) and decorin (DCN). Neither uncoated nor biofunctionalized TPCU scaffolds induced major adverse immune responses except for minor signs of polymorph nuclear cell activation. The in vivo endothelial progenitor cell homing potential of the biofunctionalized scaffolds was simulated in vitro by attracting endothelial colony-forming cells (ECFCs). Although DCN coating did attract ECFCs in combination with FN (FN + DCN), DCN-coated TPCU scaffolds showed a cell-repellent effect in the absence of FN. In a tissue-engineering approach, the electrospun and biofunctionalized tubular grafts were cultured with primary-isolated vascular endothelial cells in a custom-made bioreactor under dynamic conditions with the aim to engineer an advanced therapy medicinal product. Both FN and FN + DCN functionalization supported the formation of a confluent and functional endothelial layer.
KW  - vascular graft
KW  - endothelialization
KW  - tissue engineering
KW  - decorin
KW  - fibronectin
KW  - electrospinning
KW  - endothelial progenitor cells
KW  - bioreactor
KW  - biostable polyurethane
Y1  - 2020
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-30580
SN  - 2073-4409
SS  - 2073-4409
U6  - https://doi.org/10.3390/cells9030778
DO  - https://doi.org/10.3390/cells9030778
VL  - 9
IS  - 3
SP  - 1
EP  - 29
S1  - 29
PB  - MDPI
CY  - Basel
ER  -