TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Videv, Pavel
A1  - Mladenov, Nikola
A1  - Andreeva, Tonya
A1  - Mladenova, Kirilka
A1  - Moskova-Doumanova, Veselina
A1  - Nikolaev, Georgi
A1  - Petrova, Svetla D.
A1  - Doumanov, Jordan A.
T1  - Condensing effect of cholesterol on hBest1/POPC and hBest1/SM Langmuir monolayers
JF  - Membranes
N2  - Human bestrophin-1 protein (hBest1) is a transmembrane channel associated with the calcium-dependent transport of chloride ions in the retinal pigment epithelium as well as with the transport of glutamate and GABA in nerve cells. Interactions between hBest1, sphingomyelins, phosphatidylcholines and cholesterol are crucial for hBest1 association with cell membrane domains and its biological functions. As cholesterol plays a key role in the formation of lipid rafts, motional ordering of lipids and modeling/remodeling of the lateral membrane structure, we examined the effect of different cholesterol concentrations on the surface tension of hBest1/POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) and hBest1/SM Langmuir monolayers in the presence/absence of Ca2+ ions using surface pressure measurements and Brewster angle microscopy studies. Here, we report that cholesterol: (1) has negligible condensing effect on pure hBest1 monolayers detected mainly in the presence of Ca2+ ions, and; (2) induces a condensing effect on composite hBest1/POPC and hBest1/SM monolayers. These results offer evidence for the significance of intermolecular protein–lipid interactions for the conformational dynamics of hBest1 and its biological functions as multimeric ion channel.
KW  - hBest1
KW  - cholesterol
KW  - POPC
KW  - Langmuir monolayers
KW  - condensing effect
KW  - sphingomyelin
Y1  - 2021
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-32422
SN  - 2077-0375
SS  - 2077-0375
U6  - https://doi.org/10.3390/membranes11010052
DO  - https://doi.org/10.3390/membranes11010052
VL  - 11
IS  - 1
SP  - 8
S1  - 8
PB  - MDPI
CY  - Basel
ER  -