TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Nellinger, Svenja
A1  - Mrsic, Ivana
A1  - Keller, Silke
A1  - Heine, Simon
A1  - Southan, Alexander
A1  - Bach, Monika
A1  - Volz, Ann-Cathrin
A1  - Chassé, Thomas
A1  - Kluger, Petra
T1  - Cell-derived and enzyme-based decellularized extracellular matrix exhibit compositional and structural differences that are relevant for its use as a biomaterial
JF  - Biotechnology and bioengineering
N2  - Due to its availability and minimal invasive harvesting human adipose tissue-derived extracellular matrix (dECM) is often used as a biomaterial in various tissue engineering and healthcare applications. Next to dECM, cell-derived ECM (cdECM) can be generated by and isolated from in vitro cultured cells. So far both types of ECM were investigated extensively toward their application as (bio)material in tissue engineering and healthcare. However, a systematic characterization and comparison of soft tissue dECM and cdECM is still missing. In this study, we characterized dECM from human adipose tissue, as well as cdECM from human adipose-derived stem cells, toward their molecular composition, structural characteristics, and biological purity. The dECM was found to exhibit higher levels of collagens and lower levels of sulfated glycosaminoglycans compared with cdECMs. Structural characteristics revealed an immature state of the fibrous part of cdECM samples. By the identified differences, we aim to support researchers in the selection of a suitable ECM-based biomaterial for their specific application and the interpretation of obtained results.
Y1  - 2022
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-35974
SN  - 0006-3592
SS  - 0006-3592
U6  - https://doi.org/10.1002/bit.28047
DO  - https://doi.org/10.1002/bit.28047
VL  - 119
IS  - 4
SP  - 1142
EP  - 1156
S1  - 15
PB  - Wiley
CY  - New York
ER  -