TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Ren, Yanru
A1  - Liu, Luo
A1  - Xiong, Xin
A1  - Krastev, Rumen
A1  - Smeets, Ralf
A1  - Rimashevskiy, Denis
A1  - Schnettler, Reinhard
A1  - Alkildani, Said
A1  - Emmert, Steffen
A1  - Jung, Ole
A1  - Barbeck, Mike
T1  - Suitability of R. pulmo jellyfish-collagen-coated well plates for cytocompatibility analyses of biomaterials
JF  - International journal of molecular sciences
N2  - Cytocompatibility analyses of new implant materials or biomaterials are not only prescribed by the Medical Device Regulation (MDR), as defined in the DIN ISO Norm 10993-5 and -12, but are also increasingly replacing animal testing. In this context, jellyfish collagen has already been established as an alternative to mammalian collagen in different cell culture conditions, but a lack of knowledge exists about its applicability for cytocompatibility analyses of biomaterials. Thus, the present study was conducted to compare well plates coated with collagen type 0 derived from Rhizostoma pulmo with plates coated with bovine and porcine collagen. The coated well plates were analysed in vitro for their cytocompatibility, according to EN ISO 10993-5/−12, using both L929 fibroblasts and MC3T3 pre-osteoblasts. Thereby, the coated well plates were compared, using established materials as positive controls and a cytotoxic material, RM-A, as a negative control. L929 cells exhibited a significantly higher viability (#### p < 0.0001), proliferation (## p < 0.01), and a lower cytotoxicity (## p < 0.01 and # p < 0.05)) in the Jellagen® group compared to the bovine and porcine collagen groups. MC3T3 cells showed similar viability and acceptable proliferation and cytotoxicity in all collagen groups. The results of the present study revealed that the coating of well plates with collagen Type 0 derived from R. pulmo leads to comparable results to the case of well plates coated with mammalian collagens. Therefore, it is fully suitable for the in vitro analyses of the cytocompatibility of biomaterials or medical devices.
KW  - jellyfish collagen
KW  - biomaterials
KW  - cytocompatibility
KW  - collagen coating
KW  - well plate
KW  - medical devices
KW  - in vitro
Y1  - 2023
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-41036
SN  - 1422-0067
SS  - 1422-0067
U6  - https://doi.org/10.3390/ijms24033007
DO  - https://doi.org/10.3390/ijms24033007
VL  - 24
IS  - 3
SP  - 13
S1  - 13
PB  - MDPI
CY  - Basel
ER  -