TY - JOUR U1 - Wissenschaftlicher Artikel A1 - Surmeneva, Maria A1 - Kleinhans, Claudia A1 - Vacun, Gabriele A1 - Kluger, Petra A1 - Schönhaar, Veronika A1 - Müller, Michaela A1 - Hein, Sebastian Boris A1 - Wittmar, Alexandra A1 - Ulbricht, Mathias A1 - Prymak, Oleg A1 - Oehr, Christian A1 - Surmenev, Oleg T1 - Nano-hydroxyapatite-coated metal-ceramic composite ofiron-tricalcium Phosphate : improving the surface wettability,adhesion and proliferation of mesenchymal stem cells in vitro JF - Colloids and surfaces B : biointerfaces N2 - Thin radio-frequency magnetron sputter deposited nano-hydroxyapatite (HA) films were prepared on the surface of a Fe-tricalcium phosphate (Fe-TCP) bioceramic composite, which was obtained using a conventional powder injection moulding technique. The obtained nano-hydroxyapatite coated Fe-TCP biocomposites (nano HA-Fe-TCP) were studied with respect to their chemical and phase composition, surface morphology, water contact angle, surface free energy and hysteresis. The deposition process resulted in a homogeneous, single-phase HA coating. The ability of the surface to support adhesion and the proliferation of human mesenchymal stem cells (hMSCs) was studied using biological short-term tests in vitro. The surface of the uncoated Fe-TCP bioceramic composite showed an initial cell attachment after 24 h of seeding, but adhesion, proliferation and growth did not persist during 14 days of culture.However, the HA-Fe-TCP surfaces allowed cell adhesion, and proliferation during 14 days. The deposition of the nano-HA films on the Fe-TCP surface resulted in higher surface energy, improved hydrophilicity and biocompatibility compared with the surface of the uncoated Fe-TCP. Furthermore, it is suggested that an increase in the polar component of the surface energy was responsible for the enhanced cell adhesion and proliferation in the case of the nano-HA Fe-TCP biocomposites. KW - bioceramic composite KW - bioresorbable alloy KW - hydroxyapatite coating KW - RF magnetron sputtering KW - cell adhesion Y1 - 2015 SN - 0927-7765 SS - 0927-7765 U6 - https://doi.org/10.1016/j.colsurfb.2015.07.057 DO - https://doi.org/10.1016/j.colsurfb.2015.07.057 IS - 135 SP - 386 EP - 393 S1 - 8 PB - Elsevier CY - Amsterdam ER -