TY - JOUR U1 - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed) A1 - Elnathan, Roey A1 - Holle, Andrew A1 - Young, Jennifer A1 - George, Marina A1 - Omri, Heifler A1 - Goychuk, Andriy A1 - Frey, Erwin A1 - Kemkemer, Ralf A1 - Spatz, Joachim A1 - Kosloff, Alon A1 - Patolsky, Fernando A1 - Voelcker, Nicolas T1 - Optically transparent vertical silicon nanowire arrays for live-cell imaging JF - Journal of nanobiotechnology N2 - Programmable nano-bio interfaces driven by tuneable vertically configured nanostructures have recently emerged as a powerful tool for cellular manipulations and interrogations. Such interfaces have strong potential for ground-breaking advances, particularly in cellular nanobiotechnology and mechanobiology. However, the opaque nature of many nanostructured surfaces makes non-destructive, live-cell characterization of cellular behavior on vertically aligned nanostructures challenging to observe. Here, a new nanofabrication route is proposed that enables harvesting of vertically aligned silicon (Si) nanowires and their subsequent transfer onto an optically transparent substrate, with high efficiency and without artefacts. We demonstrate the potential of this route for efficient live-cell phase contrast imaging and subsequent characterization of cells growing on vertically aligned Si nanowires. This approach provides the first opportunity to understand dynamic cellular responses to a cell-nanowire interface, and thus has the potential to inform the design of future nanoscale cellular manipulation technologies. KW - glass substrate KW - silicon KW - live-cell phase-contrast imaging KW - cell–material interface KW - nanowires Y1 - 2021 UN - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-33450 SN - 1477-3155 SS - 1477-3155 U6 - https://doi.org/10.1186/s12951-021-00795-7 DO - https://doi.org/10.1186/s12951-021-00795-7 VL - 19 SP - 6 S1 - 6 PB - Springer CY - London ER -