TY - JOUR U1 - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed) A1 - Nellinger, Svenja A1 - Mrsic, Ivana A1 - Keller, Silke A1 - Heine, Simon A1 - Southan, Alexander A1 - Bach, Monika A1 - Volz, Ann-Cathrin A1 - Chassé, Thomas A1 - Kluger, Petra T1 - Cell-derived and enzyme-based decellularized extracellular matrix exhibit compositional and structural differences that are relevant for its use as a biomaterial JF - Biotechnology and bioengineering N2 - Due to its availability and minimal invasive harvesting human adipose tissue-derived extracellular matrix (dECM) is often used as a biomaterial in various tissue engineering and healthcare applications. Next to dECM, cell-derived ECM (cdECM) can be generated by and isolated from in vitro cultured cells. So far both types of ECM were investigated extensively toward their application as (bio)material in tissue engineering and healthcare. However, a systematic characterization and comparison of soft tissue dECM and cdECM is still missing. In this study, we characterized dECM from human adipose tissue, as well as cdECM from human adipose-derived stem cells, toward their molecular composition, structural characteristics, and biological purity. The dECM was found to exhibit higher levels of collagens and lower levels of sulfated glycosaminoglycans compared with cdECMs. Structural characteristics revealed an immature state of the fibrous part of cdECM samples. By the identified differences, we aim to support researchers in the selection of a suitable ECM-based biomaterial for their specific application and the interpretation of obtained results. Y1 - 2022 UN - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-35974 SN - 0006-3592 SS - 0006-3592 U6 - https://doi.org/10.1002/bit.28047 DO - https://doi.org/10.1002/bit.28047 VL - 119 IS - 4 SP - 1142 EP - 1156 S1 - 15 PB - Wiley CY - Hoboken, NJ ER -