TY  - JOUR
U1  - Wissenschaftlicher Artikel
A1  - Stefanakis, Mona
A1  - Bassler, Miriam
A1  - Walczuch, Tobias
A1  - Gerhard-Hartmann, Elena
A1  - Youssef, Almoatazbellah
A1  - Scherzad, Agmal
A1  - Stöth, Manuel
A1  - Ostertag, Edwin
A1  - Hagen, Rudolf
A1  - Steinke, Maria
A1  - Hackenberg, Stephan
A1  - Brecht, Marc
A1  - Meyer, Till
T1  - The impact of tissue preparation on salivary gland tumors investigated by Fourier-transform infrared microspectroscopy
JF  - Journal of clinical medicine
N2  - Due to the wide variety of benign and malignant salivary gland tumors, classification and malignant behavior determination based on histomorphological criteria can be difficult and sometimes impossible. Spectroscopical procedures can acquire molecular biological information without destroying the tissue within the measurement processes. Since several tissue preparation procedures exist, our study investigated the impact of these preparations on the chemical composition of healthy and tumorous salivary gland tissue by Fourier-transform infrared (FTIR) microspectroscopy. Sequential tissue cross-sections were prepared from native, formalin-fixed and formalin-fixed paraffin-embedded (FFPE) tissue and analyzed. The FFPE cross-sections were dewaxed and remeasured. By using principal component analysis (PCA) combined with a discriminant analysis (DA), robust models for the distinction of sample preparations were built individually for each parotid tissue type. As a result, the PCA-DA model evaluation showed a high similarity between native and formalin-fixed tissues based on their chemical composition. Thus, formalin-fixed tissues are highly representative of the native samples and facilitate a transfer from scientific laboratory analysis into the clinical routine due to their robust nature. Furthermore, the dewaxing of the cross-sections entails the loss of molecular information. Our study successfully demonstrated how FTIR microspectroscopy can be used as a powerful tool within existing clinical workflows.
KW  - formalin
KW  - fixation
KW  - tissue preparation
KW  - salivary gland neoplasia
KW  - FTIR spectroscopy
KW  - principal component analysis
KW  - discriminant analysis
Y1  - 2023
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-41539
SN  - 2077-0383
SS  - 2077-0383
U6  - https://doi.org/10.3390/jcm12020569
DO  - https://doi.org/10.3390/jcm12020569
VL  - 12
IS  - 2
SP  - 1
EP  - 17
S1  - 17
PB  - MDPI
CY  - Basel
ER  -