TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Albrecht, Franziska
A1  - Schmidt, Freia
A1  - Schmidt, Christian
A1  - Börret, Rainer
A1  - Kluger, Petra
T1  - Robot-based 6D bioprinting for soft tissue biomedical applications
JF  - Engineering in life sciences
N2  - Within this interdisciplinary study, we demonstrate the applicability of a 6D printer for soft tissue engineering models. For this purpose, a special plant was constructed, combining the technical requirements for 6D printing with the biological necessities, especially for soft tissue. Therefore, a commercial 6D robot arm was combined with a sterilizable housing (including a high-efficiency particulate air (HEPA) filter and ultraviolet radiation (UVC) lamps) and a custom-made printhead and printbed. Both components allow cooling and heating, which is desirable for working with viable cells. In addition, a spraying unit was installed that allows the distribution of fine droplets of a liquid. Advanced geometries on uneven or angled surfaces can be created with the use of all six axes. Based on often used bioinks in the field of soft tissue engineering (gellan gum, collagen, and gelatin methacryloyl) with very different material properties, we could demonstrate the flexibility of the printing system. Furthermore, cell-containing constructs using primary human adipose-derived stem cells (ASCs) could be produced in an automated manner. In addition to cell survival, the ability to differentiate along the adipogenic lineage could also be demonstrated as a representative of soft tissue engineering.
KW  - 6D bioprinting
KW  - human stem cells
KW  - robot arm
KW  - soft tissue engineering
KW  - bioinks
Y1  - 2024
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-50490
SN  - 1618-2863
SS  - 1618-2863
U6  - https://doi.org/10.1002/elsc.202300226
DO  - https://doi.org/10.1002/elsc.202300226
VL  - 24
IS  - 7
SP  - 1
EP  - 11
S1  - 11
PB  - Wiley
CY  - Hoboken, NJ
ER  -