TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Fink, Tobias
A1  - Wolf, Alexander
A1  - Maurer, Felix
A1  - Albrecht, Frederic
A1  - Heim, Nathalie
A1  - Wolf, Beate
A1  - Hauschild, Anne-Christin
A1  - Bödeker, Bertram
A1  - Baumbach, Jörg
A1  - Volk, Thomas
A1  - Sessler, Daniel
A1  - Kreuer, Sascha
T1  - Volatile organic compounds during inflammation and sepsis in rats : a potential breath test using ion-mobility spectrometry
JF  - Anesthesiology : the journal of the American Society of Anesthesiologists
N2  - Background: Multicapillary column ion-mobility spectrometry (MCC-IMS) may identify volatile components in exhaled gas. The authors therefore used MCC-IMS to evaluate exhaled gas in a rat model of sepsis, inflammation, and hemorrhagic shock.

Methods: Male Sprague-Dawley rats were anesthetized and ventilated via tracheostomy for 10 h or until death. Sepsis was induced by cecal ligation and incision in 10 rats; a sham operation was performed in 10 others. In 10 other rats, endotoxemia was induced by intravenous administration of 10 mg/kg lipopolysaccharide. In a final 10 rats, hemorrhagic shock was induced to a mean arterial pressure of 35 +/- 5 mmHg. Exhaled gas was analyzed with MCC-IMS, and volatile compounds were identified using the BS-MCC/IMS-analytes database (Version 1209; B&S Analytik, Dortmund, Germany).

Results: All sham animals survived the observation period, whereas mean survival time was 7.9 h in the septic animals, 9.1 h in endotoxemic animals, and 2.5 h in hemorrhagic shock. Volatile compounds showed statistically significant differences in septic and endotoxemic rats compared with sham rats for 3-pentanone and acetone. Endotoxic rats differed significantly from sham for 1-propanol, butanal, acetophenone, 1,2-butandiol, and 2-hexanone. Statistically significant differences were observed between septic and endotoxemic rats for butanal, 3-pentanone, and 2-hexanone. 2-Hexanone differed from all other groups in the rats with shock.

Conclusions: Breath analysis of expired organic compounds differed significantly in septic, inflammation, and sham rats. MCC-IMS of exhaled breath deserves additional study as a noninvasive approach for distinguishing sepsis from inflammation.
Y1  - 2015
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-64
SN  - 0003-3022
SS  - 0003-3022
U6  - https://doi.org/10.1097/ALN.0000000000000420
DO  - https://doi.org/10.1097/ALN.0000000000000420
VL  - 122
IS  - 1
SP  - 117
EP  - 126
S1  - 9
PB  - Lippincott Williams & Wilkins
CY  - Hagerstown
ER  -