TY - JOUR U1 - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed) A1 - Uharek, Samira A1 - Baratchi, Sara A1 - Zhu, Jiu Yang A1 - Alshehri, Majed Ali A1 - Mitchell, Arnan A1 - Rebner, Karsten A1 - Christian, Karnutsch A1 - Khoshmanesh, Khashayar T1 - Water jacket systems for temperature control of Petri dish cell culture chambers JF - Applied Sciences N2 - Water jacket systems are routinely used to control the temperature of Petri dish cell culture chambers. Despite their widespread use, the thermal characteristics of such systems have not been fully investigated. In this study, we conducted a comprehensive set of theoretical, numerical and experimental analyses to investigate the thermal characteristics of Petri dish chambers under stable and transient conditions. In particular, we investigated the temperature gradient along the radial axis of the Petri dish under stable conditions, and the transition period under transient conditions. Our studies indicate a radial temperature gradient of 3.3 °C along with a transition period of 27.5 min when increasing the sample temperature from 37 to 45 °C for a standard 35 mm diameter Petri dish. We characterized the temperature gradient and transition period under various operational, geometric, and environmental conditions. Under stable conditions, reducing the diameter of the Petri dish and incorporating a heater underneath the Petri dish can effectively reduce the temperature gradient across the sample. In comparison, under transient conditions, reducing the diameter of the Petri dish, reducing sample volume, and using glass Petri dish chambers can reduce the transition period. KW - temperature control KW - water jacket KW - convective heaters KW - Petri dish Y1 - 2019 UN - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-20678 SN - 2076-3417 SS - 2076-3417 U6 - https://doi.org/10.3390/app9040621 DO - https://doi.org/10.3390/app9040621 VL - 9 IS - 4 SP - 1 EP - 18 S1 - 18 PB - MDPI CY - Basel ER -