TY - JOUR U1 - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed) A1 - Barbeck, Mike A1 - Jung, Ole A1 - Xiong, Xin A1 - Krastev, Rumen A1 - Korzinskas, Tadas A1 - Najman, Stevo T1 - Balancing purification and ultrastructure of naturally derived bone blocks for bone regeneration: report of the purification effort of two bone blocks JF - Materials / Molecular Diversity Preservation International N2 - The present publication reports the purification effort of two natural bone blocks, that is, an allogeneic bone block (maxgraft®, botiss biomaterials GmbH, Zossen, Germany) and a xenogeneic block (SMARTBONE®, IBI S.A., Mezzovico Vira, Switzerland) in addition to previously published results based on histology. Furthermore, specialized scanning electron microscopy (SEM) and in vitro analyses (XTT, BrdU, LDH) for testing of the cytocompatibility based on ISO 10993-5/-12 have been conducted. The microscopic analyses showed that both bone blocks possess a trabecular structure with a lamellar subarrangement. In the case of the xenogeneic bone block, only minor remnants of collagenous structures were found, while in contrast high amounts of collagen were found associated with the allogeneic bone matrix. Furthermore, only island-like remnants of the polymer coating in case of the xenogeneic bone substitute seemed to be detectable. Finally, no remaining cells or cellular remnants were found in both bone blocks. The in vitro analyses showed that both bone blocks are biocompatible. Altogether, the purification level of both bone blocks seems to be favorable for bone tissue regeneration without the risk for inflammatory responses or graft rejection. Moreover, the analysis of the maxgraft® bone block showed that the underlying purification process allows for preserving not only the calcified bone matrix but also high amounts of the intertrabecular collagen matrix. KW - bone block KW - allogeneic KW - xenogeneic KW - purification KW - bone regeneration KW - dentistry Y1 - 2019 UN - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-25477 SN - 1996-1944 SS - 1996-1944 U6 - https://doi.org/10.3390/ma12193234 DO - https://doi.org/10.3390/ma12193234 VL - 12 IS - 19 SP - 1 EP - 13 S1 - 13 PB - MDPI CY - Basel ER -