TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Metzger, Michael
A1  - Konrad, Alexander
A1  - Skandary, Sepideh
A1  - Ashraf, Imran
A1  - Meixner, Alfred
A1  - Brecht, Marc
T1  - Resolution enhancement for low-temperature scanning microscopy by cryo-immersion
JF  - Optics express
N2  - Here we report a simple way to enhance the resolution of a confocal scanning microscope under cryogenic conditions. Using a microscope objective (MO) with high numerical aperture (NA = 1:25) and 1-propanol as an immersion fluid with low freezing temperature we were able to reach an imaging resolution at 160 K comparable to ambient conditions. The MO and the sample were both placed inside the inner chamber of the cryostat to reduce distortions induced by temperature gradients. The image quality of our commercially available MO was further enhanced by scanning the sample (sample scanning) in contrast to beam scanning. The ease of the whole procedure marks an essential step towards the development of cryo high-resolution microscopy and correlative light and electron cryo microscopy (cryoCLEM).
KW  - confocal microscopy
KW  - fluorescence microscopy
KW  - scanning microscopy
KW  - spatial resolution
Y1  - 2016
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-10282
SN  - 1094-4087
SS  - 1094-4087
U6  - https://doi.org/10.1364/OE.24.013023
DO  - https://doi.org/10.1364/OE.24.013023
VL  - 24
IS  - 12
SP  - 13023
EP  - 13032
S1  - 10
PB  - Optical Society of America
CY  - Washington, DC
ER  -