TY - JOUR U1 - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed) A1 - Ashraf, Imran A1 - Konrad, Alexander A1 - Lokstein, Heiko A1 - Skandary, Sepideh A1 - Metzger, Michael A1 - Djouda, Joseph A1 - Maurer, Thomas A1 - Adam, Pierre-Michel A1 - Meixner, Alfred A1 - Brecht, Marc T1 - Temperature dependence of metal-enhanced fluorescence of photosystem I from Thermosynechococcus elongatus JF - Nanoscale N2 - We report the temperature dependence of metal-enhanced fluorescence (MEF) of individual photosystem I (PSI) complexes from Thermosynechococcus elongatus (T. elongatus) coupled to gold nanoparticles (AuNPs). A strong temperature dependence of shape and intensity of the emission spectra is observed when PSI is coupled to AuNPs. For each temperature, the enhancement factor (EF) is calculated by comparing the intensity of individual AuNP-coupled PSI to the mean intensity of ‘uncoupled’ PSI. At cryogenic temperature (1.6 K) the average EF was 4.3-fold. Upon increasing the temperature to 250 K the EF increases to 84-fold. Single complexes show even higher EFs up to 441.0-fold. At increasing temperatures the different spectral pools of PSI from T. elongatus become distinguishable. These pools are affected differently by the plasmonic interactions and show different enhancements. The remarkable increase of the EFs is explained by a rate model including the temperature dependence of the fluorescence yield of PSI and the spectral overlap between absorption and emission spectra of AuNPs and PSI, respectively. Y1 - 2017 SN - 2040-3364 SS - 2040-3364 U6 - https://doi.org/10.1039/C6NR08762K DO - https://doi.org/10.1039/C6NR08762K VL - 9 IS - 12 SP - 4196 EP - 4204 S1 - 9 PB - Royal Society of Chemistry CY - Cambridge ER -