TY - JOUR U1 - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed) A1 - Häussling, Victor A1 - Deninger, Sebastian A1 - Vidoni, Laura A1 - Rinderknecht, Helen A1 - Ruoß, Marc A1 - Arnscheidt, Christian A1 - Athanasopulu, Kiriaki A1 - Kemkemer, Ralf T1 - Impact of four protein additives in cryogels on osteogenic differentiation of adipose-derived mesenchymal stem cells JF - Bioengineering : open access journal N2 - Human adipose-derived mesenchymal stem/stromal cells (Ad-MSCs) have great potential for bone tissue engineering. Cryogels, mimicking the three-dimensional structure of spongy bone, represent ideal carriers for these cells. We developed poly(2-hydroxyethyl methacrylate) cryogels, containing hydroxyapatite to mimic inorganic bone matrix. Cryogels were additionally supplemented with different types of proteins, namely collagen (Coll), platelet rich plasma (PRP), immune cells-conditioned medium (CM), and RGD peptides (RGD). The different protein components did not affect scaffolds’ porosity or water-uptake capacity, but altered pore size and stiffness. Stiffness was highest in scaffolds with PRP (82.3 kPa), followed by Coll (55.3 kPa), CM (45.6 kPa), and RGD (32.8 kPa). Scaffolds with PRP, CM, and Coll had the largest pore diameters (~60 µm). Ad MSCs were osteogenically differentiated on these scafffolds for 14 days. Cell attachment and survival rates were comparable for all four scaffolds. Runx2 and osteocalcin levels only increased in Ad-MSCs on Coll, PRP and CM cryogels. Osterix levels increased slightly in Ad-MSCs differentiated on Coll and PRP cryogels. With differentiation alkaline phosphatase activity decreased under all four conditions. In summary, besides Coll cryogel our PRP cryogel constitutes as an especially suitable carrier for bone tissue engineering. This is of special interest, as this scaffold can be generated with patients’ PRP. KW - bone tissue engineering KW - cryogel KW - adipose-derived mesenchymal stem/stromal cells KW - 3D-culture KW - scaffold KW - platelet-rich plasma (PRP) KW - RGD KW - collagen KW - immune-cell conditioned medium Y1 - 2019 UN - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-23856 SN - 2306-5354 SS - 2306-5354 U6 - https://doi.org/10.3390/bioengineering6030067 DO - https://doi.org/10.3390/bioengineering6030067 VL - 6 IS - 3 SP - 1 EP - 20 S1 - 20 PB - MDPI CY - Basel ER -