TY - JOUR U1 - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed) A1 - Greiner, Alexandra A1 - Hoffmann, Peter A1 - Bruellhoff, Kristina A1 - Jungbauer, Simon A1 - Spatz, Joachim A1 - Moeller, Martin A1 - Kemkemer, Ralf A1 - Groll, Jürgen T1 - Stable biochemically micro-patterned hydrogel layers control specific cell adhesion and allow long term cyclic tensile strain experiments JF - Macromolecular bioscience N2 - Poly(dimethylsiloxane) can be covalently coated with ultrathin NCO-sP(EO-stat-PO) hydrogel layers which permit covalent binding of cell adhesive moieties, while minimizing unspecific cell adhesion on non-functionalized areas. We applied long term uniaxial cyclic tensile strain (CTS) and revealed (a) the preservation of protein and cell-repellent properties of the NCO-sP(EO-stat-PO) coating and (b) the stability and bioactivity of a covalently bound fibronectin (FN) line pattern. We studied the adhesion of human dermal fibroblast (HDFs) on non-modified NCO-sP(EO-stat-PO) coatings and on the FN. HDFs adhered to FN and oriented their cell bodies and actin fibers along the FN lines independently of the direction of CTS. This mechanical long term stability of the bioactive, patterned surface allows unraveling biomechanical stimuli for cellular signaling and behavior to understand physiological and pathological cell phenomenon. Additionally, it allows for the application in wound healing assays, tissue engineering, and implant development demanding spatial control over specific cell adhesion. KW - hydrogels KW - mechanical properties KW - micro-contact printing of fibronectin KW - specific cell adhesion KW - spin coating of star polymers Y1 - 2014 SN - 1616-5187 SS - 1616-5187 U6 - https://doi.org/10.1002/mabi.201400261 DO - https://doi.org/10.1002/mabi.201400261 VL - 14 IS - 11 SP - 1547 EP - 1555 S1 - 9 PB - Wiley CY - Weinheim ER -