TY - JOUR U1 - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed) A1 - Frey, Christoph A1 - Sales, Adria A1 - Athanasopulu, Kiriaki A1 - Spatz, Joachim A1 - Kemkemer, Ralf T1 - Hydrogels with precisely nano-functionalized micro-topography for cell guidance JF - Biomedizinische Technik / Biomedical engineering N2 - In vivo, cells encounter different physical and chemical signals in the extracellular matrix (ECM) which regulate their behavior. Examples of these signals are micro- and nanometer-sized features, the rigidity, and the chemical composition of the ECM. The study of cell responses to such cues is important to understand complex cell functions, some diseases, and is basis for the development of new biomaterials for applications in medical implants or regenerative medicine. Therefore, the development of new methods for surface modifications with controlled physical and chemical features is crucial. In this work, we report a new combination of micelle nanolithography (BCML) and soft micro-lithography, for the production of polyethylene glycol (PEG) hydrogels, with a micro-grooved surface and decoration with hexagonally precisely arranged gold nanoparticles (AU NPs). The Au-NPs are used for binding adhesive ligands in a well-defined density. First tests were performed by culturing human fibroblasts on the gels. Adhesion and alignment of the cells along the parallel grooves of the surface were investigated. The substrates could provide a new platform for studying cell contact guidance by micro structures, and may enable a more precise control of cell behavior by nanometrically controlled surface functionalization. Y1 - 2014 UN - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-755 SN - 0013-5585 SS - 0013-5585 U6 - https://doi.org/10.1515/bmt-2014-5000 DO - https://doi.org/10.1515/bmt-2014-5000 VL - 59 IS - s1 : Track A - Biomaterials and Biocompatibility SP - S6 EP - S9 S1 - 4 PB - de Gruyter CY - Berlin ER -