TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Huber, Birgit
A1  - Engelhardt, Sascha
A1  - Meyer, Wolfdietrich
A1  - Krüger, Hartmut
A1  - Wenz, Annika
A1  - Schönhaar, Veronika
A1  - Tovar, Günter
A1  - Kluger, Petra
A1  - Borchers, Kirsten
T1  - Blood-vessel mimicking structures by stereolithographic fabrication of small porous tubes using cytocompatible polyacrylate elastomers, biofunctionalization and endothelialization
JF  - Journal of functional biomaterials
N2  - Blood vessel reconstruction is still an elusive goal for the development of in vitro models as well as artificial vascular grafts. In this study, we used a novel photo curable cytocompatible polyacrylate material (PA) for freeform generation of synthetic vessels. We applied stereolithography for the fabrication of arbitrary 3D tubular structures with total dimensions in the centimeter range, 300 µm wall thickness, inner diameters of 1 to 2 mm and defined pores with a constant diameter of approximately 100 µm or 200 µm. We established a rinsing protocol to remove remaining cytotoxic substances from the photo-cured PA and applied thio-modified heparin and RGDC-peptides to functionalize the PA surface for enhanced endothelial cell adhesion. A rotating seeding procedure was introduced to ensure homogenous endothelial monolayer formation at the inner luminal tube wall. We showed that endothelial cells stayed viable and adherent and aligned along the medium flow under fluid-flow conditions comparable to native capillaries. The combined technology approach comprising of freeform additive manufacturing (AM), biomimetic design, cytocompatible materials which are applicable to AM, and biofunctionalization of AM constructs has been introduced as BioRap® technology by the authors.
KW  - stereolithography
KW  - artificial blood vessel
KW  - small branched and porous tubes
KW  - biofunctionalization
KW  - thio-modified heparin
KW  - endothelialization
Y1  - 2016
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-13369
SN  - 2079-4983
SS  - 2079-4983
U6  - https://doi.org/10.3390/jfb7020011
DO  - https://doi.org/10.3390/jfb7020011
VL  - 7
IS  - 2
SP  - 1
EP  - 18
S1  - 18
PB  - MDPI
CY  - Basel
ER  -