TY - JOUR U1 - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed) A1 - Huber, Birgit A1 - Hoch, Eva A1 - Calderon, Iván A1 - Borchers, Kirsten A1 - Kluger, Petra T1 - A versatile perfusion bioreactor and endothelializable photo cross-linked tubes of gelatin methacryloyl as promising tools in tissue engineering JF - Biomedical engineering = Biomedizinische Technik N2 - Size and function of bioartificial tissue models are still limited due to the lack of blood vessels and dynamic perfusion for nutrient supply. In this study, we evaluated the use of cytocompatible methacryl-modified gelatin for the fabrication of a hydrogel-based tube by dip-coating and subsequent photo-initiated cross-linking. The wall thickness of the tubes and the diameter were tuned by the degree of gelatin methacryl-modification and the number of dipping cycles. The dipping temperature of the gelatin solution was adjusted to achieve low viscous fluids of approximately 0.1 Pa s and was different for gelatin derivatives with different modification degrees. A versatile perfusion bioreactor for the supply of surrounding tissue models was developed, which can be adaped to several geometries and sizes of blood-vessel mimicking tubes. The manufactured bendable gelatin tubes were permeable for water and dissolved substances, like Nile Blue and serum albumin. As a proof of concept, human fibroblasts in a three-dimensional collagen tissue model were sucessfully supplied with nutrients via the central gelatin tube under dynamic conditions for 2 days. Moreover, the tubes could be used as scaffolds to build-up a functional and viable endothelial layer. Hence, the presented tools can contribute to solving current challenges in tissue engineering. KW - dip-coating KW - endothelial cells KW - fibroblasts KW - methacryl-modified gelatin KW - perfusion bioreactor KW - tubular perfusion system KW - vascularized tissue engineering Y1 - 2019 SN - 0013-5585 SS - 0013-5585 U6 - https://doi.org/10.1515/bmt-2018-0015 DO - https://doi.org/10.1515/bmt-2018-0015 VL - 64 IS - 4 SP - 397 EP - 406 S1 - 10 PB - De Gruyter CY - Berlin ER -