TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Wenz, Annika
A1  - Tjoeng, Iva
A1  - Schneider, Isabelle
A1  - Kluger, Petra
A1  - Borchers, Kirsten
T1  - Improved vasculogenesis and bone matrix formation through coculture of endothelial cells and stem cells in tissue‐specific methacryloyl gelatin‐based hydrogels
JF  - Biotechnology & bioengineering
N2  - The coculture of osteogenic and angiogenic cells and the resulting paracrine signaling via soluble factors are supposed to be crucial for successfully engineering vascularized bone tissue equivalents. In this study, a coculture system combining primary human adiposederived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl‐modified gelatin (GM) as three‐dimensional scaffolds was examined for its support of tissue specific cell functions. HDMECs, together with hASCs as supporting cells, were encapsulated in soft GM gels and were indirectly cocultured with hASCs encapsulated in stiffer GM hydrogels additionally containing methacrylate‐modified hyaluronic acid and hydroxyapatite particles. After 14 days, the hASC in the stiffer gels (constituting the “bone gels”) expressed matrix proteins like collagen type I and fibronectin, as well as bone‐specific proteins osteopontin and alkaline phosphatase. After 14 days of coculture with HDMEC‐laden hydrogels, the viscoelastic properties of the bone gels were significantly higher compared with the gels in monoculture. Within the soft vascularization gels, the formed capillary‐like networks were significantly longer after 14 days of coculture than the structures in the control gels. In addition, the stability as well as the complexity of the vascular networks was significantly increased by coculture. We discussed and concluded that osteogenic and angiogenic signals from the culture media as well as from cocultured cell types, and tissue‐specific hydrogel composition all contribute to stimulate the interplay between osteogenesis and angiogenesis in vitro and are a basis for engineering vascularized bone.
KW  - bone matrix formation
KW  - capillary formation
KW  - coculture
KW  - human adipose-derived stem cells (hASCs)
KW  - human dermal microvascular endothelial cells (HDMECs)
KW  - tissue-specific methacryoloyl-modified gelatin hydrogels
Y1  - 2018
SN  - 0006-3592
SS  - 0006-3592
U6  - https://doi.org/10.1002/bit.26792
DO  - https://doi.org/10.1002/bit.26792
VL  - 115
IS  - 10
SP  - 2643
EP  - 2653
S1  - 11
PB  - Wiley
CY  - New York, NY
ER  -