TY - JOUR U1 - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed) A1 - Keller, Silke A1 - Bakker, Tomke A1 - Kimmel, Benjamin A1 - Rebers, Lisa A1 - Götz, Tobias A1 - Tovar, Günter A1 - Kluger, Petra A1 - Southan, Alexander T1 - Azido‐functionalized gelatin via direct conversion of lysine amino groups by diazo transfer as a building block for biofunctional hydrogels JF - Journal of biomedical materials research, Part A N2 - Gelatin is one of the most prominent biopolymers in biomedical material research and development. It is frequently used in hybrid hydrogels, which combine the advantageous properties of bio‐based and synthetic polymers. To prevent the biological component from leaching out of the hydrogel, the biomolecules can be equipped with azides. Those groups can be used to immobilize gelatin covalently in hydrogels by the highly selective and specific azide–alkyne cycloaddition. In this contribution, we functionalized gelatin with azides at its lysine residues by diazo transfer, which offers the great advantage of only minimal side‐chain extension. Approximately 84–90% of the amino groups are modified as shown by 1H‐NMR spectroscopy, 2,4,6‐trinitrobenzenesulfonic acid assay as well as Fourier‐transform infrared spectroscopy, rheology, and the determination of the isoelectric point. Furthermore, the azido‐functional gelatin is incorporated into hydrogels based on poly(ethylene glycol) diacrylate (PEG‐DA) at different concentrations (0.6, 3.0, and 5.5%). All hydrogels were classified as noncyctotoxic with significantly enhanced cell adhesion of human fibroblasts on their surfaces compared to pure PEG‐DA hydrogels. Thus, the new gelatin derivative is found to be a very promising building block for tailoring the bioactivity of materials. KW - biocompatibility KW - biopolymers KW - functional materials KW - hydrogels KW - tissue engineering Y1 - 2021 UN - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-29133 SN - 1549-3296 SS - 1549-3296 U6 - https://doi.org/10.1002/jbm.a.37008 DO - https://doi.org/10.1002/jbm.a.37008 VL - 109 IS - 1 SP - 77 EP - 91 S1 - 15 PB - Wiley CY - New York ER -