TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Wenz, Annika
A1  - Borchers, Kirsten
A1  - Tovar, Günter
A1  - Kluger, Petra
T1  - Bone matrix production in hydroxyapatite-modified hydrogels suitable for bone bioprinting
JF  - Biofabrication
N2  - Though bioprinting is a forward-looking approach in bone tissue engineering, the development of bioinks which are on the one hand processable with the chosen printing technique, and on the other hand possess the relevant mechanical as well as osteoconductive features remains a challenge. In the present study, polymer solutions based on methacrylated gelatin and methacrylated hyaluronic acid modified with hydroxyapatite (HAp) particles (5 wt%) were prepared. Encapsulation of primary human adipose derived stem cells in the HAp-containing gels and culture for 28 d resulted in a storage moduli significantly increased to 126% ± 9.6% compared to the value on day 1 by the sole influence of the HAp. Additional use of osteogenic media components resulted in an increase of storage module up to 199% ± 27.8%. Similarly, the loss moduli was increased to 370% ± 122.1% under the influence of osteogenic media components and HAp. Those changes in rheological material characteristics indicate a distinct change in elastic and viscous hydrogel properties, and are attributed to extensive matrix production in the hydrogels by the encapsulated cells, what could also be proven by staining of bone matrix components like collagen I, fibronectin, alkaline phosphatase and osteopontin. When using the cell-laden polymer solutions as bioinks to build up relevant geometries, the ink showed excellent printability and the printed grid structure's integrity remained intact over a culture time of 28 d. Again, an intense matrix formation as well as upregulation of osteogenic markers by the encapsulated cells could be shown. In conclusion, we demonstrated that our HAp-containing bioinks and hydrogels on basis of methacrylated gelatin and hyaluronic acid are on the one hand highly suitable for the build up of relevant three-dimensional geometries with microextrusion bioprinting, and on the other hand exhibit a significant positive effect on bone matrix development and remodeling in the hydrogels, as indicated by rheological measurements and staining of bone components. This makes the developed composite hydrogels an excellent material for bone bioprinting approaches.
KW  - bioprinting
KW  - bone matrix formation
KW  - gelatin methacrylate hydrogel
KW  - osteogenic differentiation
KW  - rheology
KW  - hydroxyapatite nanoparticles
KW  - nanocomposite hydrogel
Y1  - 2017
SN  - 1758-5082
SS  - 1758-5082
U6  - https://doi.org/10.1088/1758-5090/aa91ec
DO  - https://doi.org/10.1088/1758-5090/aa91ec
VL  - 9
IS  - 4
SP  - 1
EP  - 14
S1  - 14
PB  - IOP Publ.
CY  - Bristol
ER  -