TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Holle, Andrew
A1  - Devi, Neethu Govindan Kutty
A1  - Clar, Kim
A1  - Fan, Anthony
A1  - Saif, Taher
A1  - Kemkemer, Ralf
A1  - Spatz, Joachim
T1  - Cancer cells invade confined microchannels via a self-directed mesenchymal-to-amoeboid transition
JF  - Nano letters : a journal dedicated to nanoscience and nanotechnology
N2  - Cancer cell invasion through physical barriers in the extracellular matrix (ECM) requires a complex synergy of traction force against the ECM, mechanosensitive feedback, and subsequent cytoskeletal rearrangement. PDMS microchannels were used to investigate the transition from mesenchymal to amoeboid invasion in cancer cells. Migration was faster in narrow 3 μm-wide channels than in wider 10 μm channels, even in the absence of cell-binding ECM proteins. Cells permeating narrow channels exhibited blebbing and had smooth leading edge profiles, suggesting an ECM-induced transition from mesenchymal invasion to amoeboid invasion. Live cell labeling revealed a mechanosensing period in which the cell attempts mesenchymal-based migration, reorganizes its cytoskeleton, and proceeds using an amoeboid phenotype. Rho/ROCK (amoeboid) and Rac (mesenchymal) pathway inhibition revealed that amoeboid invasion through confined environments relies on both pathways in a time- and ECM dependent manner. This demonstrates that cancer cells can dynamically modify their invasion programming to navigate physically confining matrix conditions.
KW  - cancer cell invasion
KW  - mechanobiology
KW  - microchannels
KW  - confined migration
Y1  - 2019
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-23924
SN  - 1530-6984
SS  - 1530-6984
U6  - https://doi.org/10.1021/acs.nanolett.8b04720
DO  - https://doi.org/10.1021/acs.nanolett.8b04720
VL  - 19
IS  - 4
SP  - 2280
EP  - 2290
S1  - 11
PB  - American Chemical Society
CY  - Washington, DC
ER  -