TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Rebner, Karsten
A1  - Ostertag, Edwin
A1  - Kessler, Rudolf
T1  - Hyperspectral backscatter imaging : a label-free approach to cytogenetics
JF  - Analytical and bioanalytical chemistry
N2  - Current techniques for chromosome analysis need to be improved for rapid, economical identification of complex chromosomal defects by sensitive and selective visualisation. In this paper, we present a straightforward method for characterising unstained human metaphase chromosomes. Backscatter imaging in a dark-field setup combined with visible and short near-infrared spectroscopy is used to monitor morphological differences in the distribution of the chromosomal fine structure in human metaphase chromosomes. The reasons for the scattering centres in the fine structure are explained. Changes in the scattering centres during preparation of the metaphases are discussed. FDTD simulations are presented to substantiate the experimental findings. We show that local scattering features consisting of underlying spectral modulations of higher frequencies associated with a high variety of densely packed chromatin can be represented by their scatter profiles even on a sub-microscopic level. The result is independent of the chromosome preparation and structure size. This analytical method constitutes a rapid, costeffective and label-free cytogenetic technique which can be used in a standard light microscope.
KW  - spectral karyotyping
KW  - label-free
KW  - human metaphase chromosomes
KW  - dark-field imaging
KW  - scattered light spectroscopy
KW  - visible and short near-infrared spectroscopy
Y1  - 2016
SN  - 1618-2642
SS  - 1618-2642
U6  - https://doi.org/10.1007/s00216-016-9670-1
DO  - https://doi.org/10.1007/s00216-016-9670-1
VL  - 408
IS  - 21
SP  - 5701
EP  - 5709
S1  - 9
PB  - Springer
CY  - Berlin
ER  -