TY - JOUR U1 - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed) A1 - Ruoß, Marc A1 - Rebholz, Silas A1 - Weimer, Marina A1 - Grom-Baumgarten, Carl A1 - Athanasopulu, Kiriaki A1 - Kemkemer, Ralf A1 - Käß, Hanno A1 - Ehnert, Sabrina A1 - Nussler, Andreas T1 - Development of scaffolds with adjusted stiffness for mimicking disease-related alterations of liver rigidity JF - Journal of functional biomaterials N2 - Drug-induced liver toxicity is one of the most common reasons for the failure of drugs in clinical trials and frequent withdrawal from the market. Reasons for such failures include the low predictive power of in vivo studies, that is mainly caused by metabolic differences between humans and animals, and intraspecific variances. In addition to factors such as age and genetic background, changes in drug metabolism can also be caused by disease-related changes in the liver. Such metabolic changes have also been observed in clinical settings, for example, in association with a change in liver stiffness, a major characteristic of an altered fibrotic liver. For mimicking these changes in an in vitro model, this study aimed to develop scaffolds that represent the rigidity of healthy and fibrotic liver tissue. We observed that liver cells plated on scaffolds representing the stiffness of healthy livers showed a higher metabolic activity compared to cells plated on stiffer scaffolds. Additionally, we detected a positive effect of a scaffold pre-coated with fetal calf serum (FCS)-containing media. This pre-incubation resulted in increased cell adherence during cell seeding onto the scaffolds. In summary, we developed a scaffold-based 3D model that mimics liver stiffness-dependent changes in drug metabolism that may more easily predict drug interaction in diseased livers. KW - scaffold culture KW - stiffness KW - in vitro model KW - pre-coating KW - Arg-Gly-Asp (RGD)-peptides KW - cell attachment Y1 - 2020 UN - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-30134 SN - 2079-4983 SS - 2079-4983 U6 - https://doi.org/10.3390/jfb11010017 DO - https://doi.org/10.3390/jfb11010017 VL - 11 IS - 1 SP - 1 EP - 19 S1 - 19 PB - MDPI CY - Basel ER -