TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Graf, Alexander
A1  - Claßen, Jens
A1  - Solle, Dörte
A1  - Hitzmann, Bernd
A1  - Rebner, Karsten
A1  - Hoehse, Marek
T1  - A novel LED-based 2D-fluorescence spectroscopy system for in-line monitoring of Chinese hamster ovary cell cultivations - Part I
JF  - Engineering in life sciences
N2  - A new two-dimensional fluorescence sensor system was developed for in-line monitoring of mammalian cell cultures. Fluorescence spectroscopy allows for the detection and quantification of naturally occurring intra- and extracellular fluorophores in the cell broth. The fluorescence signals correlate the the cells' current redox state and other relevant process parameters. Cell culture pretests with twelve different excitation wavelengths showed that only three wavelengths account for a vast majority of spectral variation. Accordingly, the newly developed device utilizes three high-power LEDs as excitation sources in combination with a back-thinned CCD-spectrometer for fluorescence detection.
KW  - 2D-fluorescence spectroscopy
KW  - CHO cell cultivation
KW  - in-line biprocess monitoring
KW  - metabolism monitoring
KW  - MVDA
KW  - PAT
Y1  - 2019
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-24088
SN  - 1618-0240
SS  - 1618-0240
U6  - https://doi.org/10.1002/elsc.201800149
DO  - https://doi.org/10.1002/elsc.201800149
VL  - 19
IS  - 5
SP  - 352
EP  - 362
S1  - 11
PB  - Wiley
CY  - Weinheim
ER  -