TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Keller, Silke
A1  - Wörgötter, Katharina
A1  - Liedek, Anke
A1  - Kluger, Petra
A1  - Bach, Monika
A1  - Tovar, Günter
A1  - Southan, Alexander
T1  - Azide-functional extracellular matrix coatings as a bioactive platform for bioconjugation
JF  - ACS applied materials & interfaces / American Chemical Society
N2  - In recent years, the development and application of decellularized extracellular matrices (ECMs) for use as biomaterials have grown rapidly. These cell-derived matrices (CDMs) represent highly bioactive and biocompatible materials consisting of a complex assembly of biomolecules. Even though CDMs mimic the natural microenvironment of cells in vivo very closely, they still lack specifically addressable functional groups, which are often required to tailor a biomaterial functionality by bioconjugation. To overcome this limitation, metabolic glycoengineering has emerged as a powerful tool to equip CDMs with chemical groups such as azides. These small chemical handles are known for their ability to undergo bioorthogonal click reactions, which represent a desirable reaction type for bioconjugation. However, ECM insolubility makes its processing very challenging. In this contribution, we isolated both the unmodified ECM and azide-modified clickECM by osmotic lysis. In a first step, these matrices were concentrated to remove excessive water from the decellularization step. Next, the hydrogel-like ECM and clickECM films were mechanically fragmentized, resulting in easy to pipette suspensions with fragment sizes ranging from 7.62 to 31.29 μm (as indicated by the mean d90 and d10 values). The biomolecular composition was not impaired as proven by immunohistochemistry. The suspensions were used for the reproducible generation of surface coatings, which proved to be homogeneous in terms of ECM fragment sizes and coating thicknesses (the mean coating thickness was found to be 33.2 ± 7.3 μm). Furthermore, they were stable against fluid-mechanical abrasion in a laminar flow cell. When primary human fibroblasts were cultured on the coated substrates, an increased bioactivity was observed. By conjugating the azides within the clickECM coatings with alkyne-coupled biotin molecules, a bioconjugation platform was obtained, where the biotin–streptavidin interaction could be used. Its applicability was demonstrated by equipping the bioactive clickECM coatings with horseradish peroxidase as a model enzyme.
KW  - cell-derived matrices
KW  - biotinylation
KW  - bioconjugation
KW  - clickECM
Y1  - 2020
SN  - 1944-8244
SS  - 1944-8244
U6  - https://doi.org/10.1021/acsami.0c04579
DO  - https://doi.org/10.1021/acsami.0c04579
VL  - 12
IS  - 24
SP  - 26868
EP  - 26879
S1  - 12
PB  - American Chemical Society
CY  - Washington, DC
ER  -