TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Stefanakis, Mona
A1  - Lorenz, Anita
A1  - Bartsch, Jörg
A1  - Bassler, Miriam
A1  - Wagner, Alexandra
A1  - Brecht, Marc
A1  - Pagenstecher, Axel
A1  - Schnittenhelm, Jens
A1  - Boldrini, Barbara
A1  - Hakelberg, Sabrina
A1  - Noell, Susan
A1  - Nimsky, Christopher
A1  - Tatagiba, Marcos
A1  - Ritz, Rainer
A1  - Rebner, Karsten
A1  - Ostertag, Edwin
T1  - Formalin fixation as tissue preprocessing for multimodal optical spectroscopy using the example of human brain tumour cross sections
JF  - Journal of spectroscopy
N2  - Characterization of brain tumours requires neuropathological expertise and is generally performed by histological evaluation and molecular analysis. One emerging technique to assist pathologists in future tumour diagnostics is multimodal optical spectroscopy. In the current clinical routine, tissue preprocessing with formalin is widely established and suitable for spectroscopic investigations since degradation processes impede the measurement of native tissue. However, formalin fixation results in alterations of the tissue chemistry and morphology for example by protein cross-linking. As optical spectroscopy is sensitive to these variations, we evaluate the effects of formalin fixation on multimodal brain tumour data in this proof-of-concept study. Nonfixed and formalin-fixed cross sections of different common human brain tumours were subjected to analysis of chemical variations using ultraviolet and Fourier-transform infrared microspectroscopy. Morphological changes were assessed by elastic light scattering microspectroscopy in the visible wavelength range. Data were analysed with multivariate data analysis and compared with histopathology. Tissue type classifications deduced by optical spectroscopy are highly comparable and independent from the preparation and the fixation protocol. However, formalin fixation leads to slightly better classification models due to improved stability of the tissue. As a consequence, spectroscopic methods represent an appropriate additional contrast for chemical and morphological information in neuropathological diagnosis and should be investigated to a greater extent. Furthermore, they can be included in the clinical workflow even after formalin fixation.
Y1  - 2021
UN  - https://nbn-resolving.org/urn:nbn:de:bsz:rt2-opus4-31355
SN  - 0712-4813
SS  - 0712-4813
U6  - https://doi.org/10.1155/2021/5598309
DO  - https://doi.org/10.1155/2021/5598309
VL  - 2021
SP  - 1
EP  - 14
S1  - 14
PB  - Hindawi
CY  - New York
ER  -