TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Huber, Birgit
A1  - Hoch, Eva
A1  - Calderon, Iván
A1  - Borchers, Kirsten
A1  - Kluger, Petra
T1  - A versatile perfusion bioreactor and endothelializable photo cross-linked tubes of gelatin methacryloyl as promising tools in tissue engineering
JF  - Biomedical engineering = Biomedizinische Technik
N2  - Size and function of bioartificial tissue models are still limited due to the lack of blood vessels and dynamic perfusion for nutrient supply. In this study, we evaluated the use of cytocompatible methacryl-modified gelatin for the fabrication of a hydrogel-based tube by dip-coating and subsequent photo-initiated cross-linking. The wall thickness of the tubes and the diameter were tuned by the degree of gelatin methacryl-modification and the number of dipping cycles. The dipping temperature of the gelatin solution was adjusted to achieve low viscous fluids of approximately 0.1 Pa s and was different for gelatin derivatives with different modification degrees. A versatile perfusion bioreactor for the supply of surrounding tissue models was developed, which can be adaped to several geometries and sizes of blood-vessel mimicking tubes. The manufactured bendable gelatin tubes were permeable for water and dissolved substances, like Nile Blue and serum albumin. As a proof of concept, human fibroblasts in a three-dimensional collagen tissue model were sucessfully supplied with nutrients via the central gelatin tube under dynamic conditions for 2 days. Moreover, the tubes could be used as scaffolds to build-up a functional and viable endothelial layer. Hence, the presented tools can contribute to solving current challenges in tissue engineering.
KW  - dip-coating
KW  - endothelial cells
KW  - fibroblasts
KW  - methacryl-modified gelatin
KW  - perfusion bioreactor
KW  - tubular perfusion system
KW  - vascularized tissue engineering
Y1  - 2019
VL  - 64
IS  - 4
SP  - 397
EP  - 406
S1  - 10
PB  - De Gruyter
CY  - Berlin
ER  - 
TY  - JOUR
U1  - Zeitschriftenartikel, wissenschaftlich - begutachtet (reviewed)
A1  - Wenz, Annika
A1  - Janke, Katharina
A1  - Hoch, Eva
A1  - Tovar, Günter
A1  - Borchers, Kirsten
A1  - Kluger, Petra
T1  - Hydroxyapatite-modified gelatin bioinks for bone bioprinting
JF  - Bionanomaterials : journal of functional materials, biomechanics, and tissue engineering
N2  - In bioprinting approaches, the choice of bioink plays an important role since it must be processable with the selected printing method, but also cytocompatible and biofunctional. Therefore, a crosslinkable gelatin-based ink was modified with hydroxyapatite (HAp) particles, representing the composite buildup of natural bone. The inks’ viscosity was significantly increased by the addition of HAp, making the material processable with extrusion-based methods. The storage moduli of the formed hydrogels rose significantly, depicting improved mechanical properties. A cytocompatibility assay revealed suitable ranges for photoinitiator and HAp concentrations. As a proof of concept, the modified ink was printed together with cells, yielding stable three-dimensional constructs containing a homogeneously distributed mineralization and viable cells.
KW  - additive manufacturing
KW  - mineralization
KW  - osteoblast differentiation
KW  - tissue engineering
Y1  - 2016
VL  - 17
IS  - 3-4
SP  - 179
EP  - 184
S1  - 6
PB  - De Gruyter
CY  - Berlin
ER  -