Impact of four protein additives in cryogels on osteogenic differentiation of adipose-derived mesenchymal stem cells

  • Human adipose-derived mesenchymal stem/stromal cells (Ad-MSCs) have great potential for bone tissue engineering. Cryogels, mimicking the three-dimensional structure of spongy bone, represent ideal carriers for these cells. We developed poly(2-hydroxyethyl methacrylate) cryogels, containing hydroxyapatite to mimic inorganic bone matrix. Cryogels were additionally supplemented with different types of proteins, namely collagen (Coll), platelet rich plasma (PRP), immune cells-conditioned medium (CM), and RGD peptides (RGD). The different protein components did not affect scaffolds’ porosity or water-uptake capacity, but altered pore size and stiffness. Stiffness was highest in scaffolds with PRP (82.3 kPa), followed by Coll (55.3 kPa), CM (45.6 kPa), and RGD (32.8 kPa). Scaffolds with PRP, CM, and Coll had the largest pore diameters (~60 µm). Ad MSCs were osteogenically differentiated on these scafffolds for 14 days. Cell attachment and survival rates were comparable for all four scaffolds. Runx2 and osteocalcin levels only increased in Ad-MSCs on Coll, PRP and CM cryogels. Osterix levels increased slightly in Ad-MSCs differentiated on Coll and PRP cryogels. With differentiation alkaline phosphatase activity decreased under all four conditions. In summary, besides Coll cryogel our PRP cryogel constitutes as an especially suitable carrier for bone tissue engineering. This is of special interest, as this scaffold can be generated with patients’ PRP.

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Metadaten
Name:Athanasopulu, Kiriaki; Kemkemer, Ralf
URN:urn:nbn:de:bsz:rt2-opus4-23856
DOI:https://doi.org/10.3390/bioengineering6030067
ISSN:2306-5354
Erschienen in:Bioengineering : open access journal
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Year of Publication:2019
Tag:(Ad-MSCs); 3D-culture; RGD; adipose-derived mesenchymal stem/stromal cells; bone tissue engineering; collagen; cryogel; immune-cell conditioned medium; platelet-rich plasma (PRP); scaffold
Volume:6
Issue:3, Aufsatz 67
Pagenumber:20
First Page:1
Last Page:20
Dewey Decimal Classification:570 Biowissenschaften, Biologie
Open Access:Ja
Licence (German):License Logo  Open Access