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A versatile perfusion bioreactor and endothelializable photo cross-linked tubes of gelatin methacryloyl as promising tools in tissue engineering

  • Size and function of bioartificial tissue models are still limited due to the lack of blood vessels and dynamic perfusion for nutrient supply. In this study, we evaluated the use of cytocompatible methacryl-modified gelatin for the fabrication of a hydrogel-based tube by dip-coating and subsequent photo-initiated cross-linking. The wall thickness of the tubes and the diameter were tuned by the degree of gelatin methacryl-modification and the number of dipping cycles. The dipping temperature of the gelatin solution was adjusted to achieve low viscous fluids of approximately 0.1 Pa s and was different for gelatin derivatives with different modification degrees. A versatile perfusion bioreactor for the supply of surrounding tissue models was developed, which can be adaped to several geometries and sizes of blood-vessel mimicking tubes. The manufactured bendable gelatin tubes were permeable for water and dissolved substances, like Nile Blue and serum albumin. As a proof of concept, human fibroblasts in a three-dimensional collagen tissue model were sucessfully supplied with nutrients via the central gelatin tube under dynamic conditions for 2 days. Moreover, the tubes could be used as scaffolds to build-up a functional and viable endothelial layer. Hence, the presented tools can contribute to solving current challenges in tissue engineering.

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Metadaten
Name:Kluger, Petra
DOI:https://doi.org/10.1515/bmt-2018-0015
ISSN:0013-5585
Erschienen in:Biomedical engineering = Biomedizinische Technik
Publisher:De Gruyter
Place of publication:Berlin
Document Type:Article
Language:English
Year of Publication:2019
Tag:dip-coating; endothelial cells; fibroblasts; methacryl-modified gelatin; perfusion bioreactor; tubular perfusion system; vascularized tissue engineering
Volume:64
Issue:4
Pagenumber:10
First Page:397
Last Page:406
Dewey Decimal Classification:610 Medizin, Gesundheit
Open Access:Nein