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Development of a multi-well-chip for studying 2D and 3D tumor cell migration and spheroid growth in electrical fields

  • Endogenous electrical fields play an important role in various physiological and pathological events. Yet the effects of electrical cues on processes such as wound healing, tumor development or metastasis are still rarely investigated, though it is known that direct current electrical fields can alter cell migration or proliferation in vitro. Several 2D experimental models for studying cell responses to direct current electrical fields have been presented and characterized but suitable experimental models for electrotaxis studies in 3D are rare. Here we present a novel, easy-to-produce, multi-well-based galvanotactic-chamber for the use in 2D and 3D cell experiments for investigations on the influence of electrical fields on tumor cell migration and tumor spheroid growth. Our presented system allows the simultaneous application of electrical field to cells in four chambers, either cultured on the bottom of the culture-plate (2D) or embedded in hydrogel filled channels(3D). The set-up is also suitable for, live-cell-imaging. Validation tests show stable electrical fields and high cell viabilities inside the channel. Tumor spheroids of various diameters can be exposed to direct current electrical fields up to one week.

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Metadaten
Author of HS ReutlingenNaggay, Benjamin; Schmidt, Tobias; Ende, Karen; Kemkemer, Ralf
URN:urn:nbn:de:bsz:rt2-opus4-30128
DOI:https://doi.org/10.1515/cdbme-2020-3042
ISSN:2364-5504
Erschienen in:Current directions in biomedical engineering
Publisher:De Gruyter
Place of publication:Berlin
Document Type:Article
Language:English
Year of Publication:2020
Tag:2D & 3D cell culture; cell motility; electrical field; electrotaxis; galvanotaxis; tumor cells
Volume:6
Issue:3
Page Number:4
First Page:1
Last Page:4
Article Number:20203042
DDC classes:570 Biowissenschaften, Biologie
Open Access?:Ja
Licence (German):License Logo  Creative Commons - CC BY - Namensnennung 4.0 International