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Pigmentation of white, brown and green chicken eggshells analyzed by reflectance, transmittance and fluorescence spectroscopy

  • We report on the reflectance, transmittance and fluorescence spectra (λ=200–1200nm) of four types of chicken eggshells (white, brown, light green, dark green) measured in situ without pretreatment and after ablation of 20–100 μm of the outer shell regions. The color pigment protoporphyrin IX (PPIX) is embedded in the protein phase of all four shell types as highly fluorescent monomers, in the white and light green shells additionally as non-fluorescent dimers, and in the brown and dark green shells mainly as non-fluorescent poly-aggregates. The green shell colors are formed from an approximately equimolar mixture of PPIX and biliverdin. The axial distribution of protein and color pigments were evaluated from the combined reflectances of both the outer and inner shell surfaces, as well as from the transmittances. For the data generation we used the radiative transfer model in the random walk and Kubelka-Munk approaches.

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Metadaten
Author of HS ReutlingenOstertag, Edwin; Scholz, Miriam; Klein, Julia; Rebner, Karsten
URN:urn:nbn:de:bsz:rt2-opus4-22768
DOI:https://doi.org/10.1002/open.201900154
ISSN:2191-1355
eISSN:2191-1363
Erschienen in:ChemistryOpen
Publisher:Wiley
Place of publication:Weinheim
Document Type:Journal article
Language:English
Publication year:2019
Tag:UV/VIS spectroscopy; amino acids; chicken eggshells; fluorescence spectroscopy; porphyrinoids; proteins
Volume:8
Issue:8
Page Number:10
First Page:1084
Last Page:1093
DDC classes:540 Chemie und zugeordnete Wissenschaften
Open access?:Ja
Licence (German):License Logo  Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International