Generalised image processing method for quantitative analysis of nucleus, cell and focal adhesion clusters
- Focal adhesion clusters (FAC) are dynamic and complex structures that help cells to sense physicochemical properties of their environment. Research in biomaterials, cell adhesion or cell migration often involves the visualization of FAC by fluorescence staining and microscopy, which necessitates quantitative analysis of FAC and other cell features in microscopy images using image processing. Fluorescence microscopy images of human umbilical vein endothelial cells (HUVEC) obtained at 63x magnification were quantitatively analysed using ImageJ software. A generalised algorithm for selective segmentation and morphological analysis of FAC, nucleus and cell morphology is implemented. Further, a method for discrimination of FACnear the nucleus and around the periphery is implemented using masks. Our algorithm is able to effectively quantify different morphological characteristics of cell components and shows a high sensitivity and specificity while providing a modular software implementation.
| Author of HS Reutlingen | Padmakumari Hemachandran Nair, Rajasree; Kemkemer, Ralf |
|---|---|
| URN: | urn:nbn:de:bsz:rt2-opus4-35239 |
| DOI: | https://doi.org/10.1515/cdbme-2021-2142 |
| ISSN: | 2364-5504 |
| Erschienen in: | Current directions in biomedical engineering |
| Publisher: | De Gruyter |
| Place of publication: | Berlin |
| Document Type: | Journal article |
| Language: | English |
| Publication year: | 2021 |
| Tag: | ImageJ; algorithm; fluorescence; focal adhesion clusters; location selective segmentation; masks; quantitative analysis; sensitivity; specificity |
| Volume: | 7 |
| Issue: | 2 |
| Page Number: | 4 |
| First Page: | 558 |
| Last Page: | 561 |
| DDC classes: | 570 Biowissenschaften, Biologie |
| Open access?: | Ja |
| Licence (German): |  Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |
