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Volatile organic compounds during inflammation and sepsis in rats : a potential breath test using ion-mobility spectrometry

  • Background: Multicapillary column ion-mobility spectrometry (MCC-IMS) may identify volatile components in exhaled gas. The authors therefore used MCC-IMS to evaluate exhaled gas in a rat model of sepsis, inflammation, and hemorrhagic shock. Methods: Male Sprague-Dawley rats were anesthetized and ventilated via tracheostomy for 10 h or until death. Sepsis was induced by cecal ligation and incision in 10 rats; a sham operation was performed in 10 others. In 10 other rats, endotoxemia was induced by intravenous administration of 10 mg/kg lipopolysaccharide. In a final 10 rats, hemorrhagic shock was induced to a mean arterial pressure of 35 +/- 5 mmHg. Exhaled gas was analyzed with MCC-IMS, and volatile compounds were identified using the BS-MCC/IMS-analytes database (Version 1209; B&S Analytik, Dortmund, Germany). Results: All sham animals survived the observation period, whereas mean survival time was 7.9 h in the septic animals, 9.1 h in endotoxemic animals, and 2.5 h in hemorrhagic shock. Volatile compounds showed statistically significant differences in septic and endotoxemic rats compared with sham rats for 3-pentanone and acetone. Endotoxic rats differed significantly from sham for 1-propanol, butanal, acetophenone, 1,2-butandiol, and 2-hexanone. Statistically significant differences were observed between septic and endotoxemic rats for butanal, 3-pentanone, and 2-hexanone. 2-Hexanone differed from all other groups in the rats with shock. Conclusions: Breath analysis of expired organic compounds differed significantly in septic, inflammation, and sham rats. MCC-IMS of exhaled breath deserves additional study as a noninvasive approach for distinguishing sepsis from inflammation.

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Metadaten
Author of HS ReutlingenBaumbach, Jörg Ingo
URN:urn:nbn:de:bsz:rt2-opus4-64
DOI:https://doi.org/10.1097/ALN.0000000000000420
ISSN:0003-3022
eISSN:1528-1175
Erschienen in:Anesthesiology : the journal of the American Society of Anesthesiologists
Publisher:Lippincott Williams & Wilkins
Place of publication:Hagerstown
Document Type:Article
Language:English
Year of Publication:2015
Volume:122
Issue:1
Page Number:9
First Page:117
Last Page:126
DDC classes:610 Medizin, Gesundheit
Open Access?:Ja
Licence (German):License Logo  Open Access