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Age-dependent migratory behavior of human endothelial cells revealed by substrate microtopography

  • Cell migration is part of many important in vivo biological processes and is influenced by chemical and physical factors such as substrate topography. Although the migratory behavior of different cell types on structured substrates has already been investigated, up to date it is largely unknown if specimen's age affects cell migration on structures. In this work, we investigated age-dependent migratory behavior of human endothelial cells from young (≤ 31 years old) and old (≥ 60 years old) donors on poly(dimethylsiloxane) microstructured substrates consisting of well-defined parallel grooves. We observed a decrease in cell migration velocity in all substrate conditions and in persistence length perpendicular to the grooves in cells from old donors. Nevertheless, in comparison to young cells, old cells exhibited a higher cell directionality along grooves of certain depths and a higher persistence time. We also found a systematic decrease of donor age dependent responses of cell protrusions in orientation, velocity and length, all of them decreased in old cells. These observations lead us to hypothesize a possible impairment of actin cytoskeleton network and affected actin polymerization and steering systems, caused by aging.

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Metadaten
Name:Kemkemer, Ralf
DOI:https://doi.org/10.1016/j.yexcr.2018.10.008
ISSN:0014-4827
eISSN:1090-2422
Erschienen in:Experimental cell research : ECR
Publisher:Elsevier
Document Type:Article
Language:English
Year of Publication:2019
Tag:aging; endothelial cells; microtopography; migration; protrusions
Volume:374
Issue:1
Pagenumber:11
First Page:1
Last Page:11
Dewey Decimal Classification:570 Biowissenschaften, Biologie
Open Access:Nein
Licence (German):License Logo  Lizenzbedingungen Elsevier