Volatile organic compounds during inflammation and sepsis in rats : a potential breath test using ion-mobility spectrometry
- Background: Multicapillary column ion-mobility spectrometry (MCC-IMS) may identify volatile components in exhaled gas. The authors therefore used MCC-IMS to evaluate exhaled gas in a rat model of sepsis, inflammation, and hemorrhagic shock. Methods: Male Sprague-Dawley rats were anesthetized and ventilated via tracheostomy for 10 h or until death. Sepsis was induced by cecal ligation and incision in 10 rats; a sham operation was performed in 10 others. In 10 other rats, endotoxemia was induced by intravenous administration of 10 mg/kg lipopolysaccharide. In a final 10 rats, hemorrhagic shock was induced to a mean arterial pressure of 35 +/- 5 mmHg. Exhaled gas was analyzed with MCC-IMS, and volatile compounds were identified using the BS-MCC/IMS-analytes database (Version 1209; B&S Analytik, Dortmund, Germany). Results: All sham animals survived the observation period, whereas mean survival time was 7.9 h in the septic animals, 9.1 h in endotoxemic animals, and 2.5 h in hemorrhagic shock. Volatile compounds showed statistically significant differences in septic and endotoxemic rats compared with sham rats for 3-pentanone and acetone. Endotoxic rats differed significantly from sham for 1-propanol, butanal, acetophenone, 1,2-butandiol, and 2-hexanone. Statistically significant differences were observed between septic and endotoxemic rats for butanal, 3-pentanone, and 2-hexanone. 2-Hexanone differed from all other groups in the rats with shock. Conclusions: Breath analysis of expired organic compounds differed significantly in septic, inflammation, and sham rats. MCC-IMS of exhaled breath deserves additional study as a noninvasive approach for distinguishing sepsis from inflammation.
Author of HS Reutlingen | Baumbach, Jörg Ingo |
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URN: | urn:nbn:de:bsz:rt2-opus4-64 |
DOI: | https://doi.org/10.1097/ALN.0000000000000420 |
ISSN: | 0003-3022 |
eISSN: | 1528-1175 |
Erschienen in: | Anesthesiology : the journal of the American Society of Anesthesiologists |
Publisher: | Lippincott Williams & Wilkins |
Place of publication: | Hagerstown |
Document Type: | Journal article |
Language: | English |
Publication year: | 2015 |
Volume: | 122 |
Issue: | 1 |
Page Number: | 9 |
First Page: | 117 |
Last Page: | 126 |
DDC classes: | 610 Medizin, Gesundheit |
Open access?: | Ja |
Licence (German): | Open Access |