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Methacrylated gelatin and mature adipocytes are promising components for adipose tissue engineering

  • In vitro engineering of autologous fatty tissue constructs is still a major challenge for the treatment of congenital deformities, tumor resections or high-graded burns. In this study, we evaluated the suitability of photo-crosslinkable methacrylated gelatin (GM) and mature adipocytes as components for the composition of three-dimensional fatty tissue constructs. Cytocompatibility evaluations of the GM and the photoinitiator Lithium phenyl-2,4,6 trimethylbenzoylphosphinate (LAP) showed no cytotoxicity in the relevant range of concentrations. Matrix stiffness of cell-laden hydrogels was adjusted to native fatty tissue by tuning the degree of crosslinking and was shown to be comparable to that of native fatty tissue. Mature adipocytes were then cultured for 14 days within the GM resulting in a fatty tissue construct loaded with viable cells expressing cell markers perilipin A and laminin. This work demonstrates that mature adipocytes are a highly valuable cell source for the composition of fatty tissue equivalents in vitro. Photo-crosslinkable methacrylated gelatin is an excellent tissue scaffold and a promising bioink for new printing techniques due to its biocompatibility and tunable properties.

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Metadaten
Name:Kluger, Petra
DOI:https://doi.org/10.1177/0885328215587450
ISSN:0885-3282
eISSN:1530-8022
Erschienen in:Journal of biomaterials applications
Publisher:Sage
Place of publication:London
Document Type:Article
Language:English
Year of Publication:2016
Tag:adipose tissue engineering; bioprinting; mature adipocytes; methacrylated gelatin; volume stability
Volume:30
Issue:6
Pagenumber:12
First Page:699
Last Page:710
Dewey Decimal Classification:610 Medizin, Gesundheit
Open Access:Nein
Licence (English):License Logo  Lizenzbedingungen Sage