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Comparing the use of differentiated adipose-derived stem cells and mature adipocytes to model adipose tissue in vitro

  • In vitro models of human adipose tissue may serve as beneficial alternatives to animal models to study basic biological processes, identify new drug targets, and as soft tissue implants. With this approach, we aimed to evaluate adipose-derived stem cells (ASC) and mature adipocytes (MA) comparatively for the application in the in vitro setup of adipose tissue constructs to imitate native adipose tissue physiology. We used human primary MAs and human ASCs, differentiated for 14 days, and encapsulated them in collagen type I hydrogels to build up a three-dimensional (3D) adipose tissue model. The maintenance of the models was analyzed after seven days based on a viability staining. Further, the expression of the adipocyte specific protein perilipin A and the release of leptin and glycerol were evaluated. Gene transcription profiles of models based on dASCs and MAs were analyzed with regard to native adipose tissue. Compared to MAs, dASCs showed an immature differentiation state. Further, gene transcription of MAs suggests a behavior closer to native tissue in terms of angiogenesis, which supports MAs as preferred cell type. In contrast to native adipose tissue, genes of de novo lipogenesis and tissue remodeling were upregulated in the in vitro attempts.

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Metadaten
Name:Volz, Ann-Cathrin; Kluger, Petra
DOI:https://doi.org/10.1016/j.diff.2019.09.002
ISSN:0301-4681
eISSN:1432-0436
Erschienen in:Differentiation : the journal of the International Society of Differentiation
Publisher:Elsevier
Place of publication:New York, NY
Document Type:Article
Language:English
Year of Publication:2019
Tag:3D model; adipogenic differentiated adipose-derived stem cell; adipose tissue engineering; mRNA transcription; mature adipocyte
Volume:110
Pagenumber:10
First Page:19
Last Page:28
Dewey Decimal Classification:570 Biowissenschaften, Biologie
Open Access:Nein
Licence (German):License Logo  Lizenzbedingungen Elsevier