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Improvement of a three-layered in vitro skin model for topical application of irritating substances

  • In the field of skin tissue engineering, the development of physiologically relevant in vitro skin models comprising all skin layers, namely epidermis, dermis, and subcutis, is a great challenge. Increasing regulatory requirements and the ban on animal experiments for substance testing demand the development of reliable and in vivo-like test systems, which enable high-throughput screening of substances. However, the reproducibility and applicability of in vitro testing has so far been insufficient due to fibroblast-mediated contraction. To overcome this pitfall, an advanced 3-layered skin model was developed. While the epidermis of standard skin models showed an 80% contraction, the initial epidermal area of our advanced skin models was maintained. The improved barrier function of the advanced models was quantified by an indirect barrier function test and a permeability assay. Histochemical and immunofluorescence staining of the advanced model showed well-defined epidermal layers, a dermal part with distributed human dermal fibroblasts and a subcutis with round-shaped adipocytes. The successful response of these advanced 3-layered models for skin irritation testing demonstrated the suitability as an in vitro model for these clinical tests: only the advanced model classified irritative and non-irritative substances correctly. These results indicate that the advanced set up of the 3-layered in vitro skin model maintains skin barrier function and therefore makes them more suitable for irritation testing.

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Metadaten
Author of HS ReutlingenSchmidt, Freia; Nowakowski, Sophia; Kluger, Petra
URN:urn:nbn:de:bsz:rt2-opus4-29114
DOI:https://doi.org/10.3389/fbioe.2020.00388
ISSN:2296-4185
Erschienen in:Frontiers in Bioengineering and Biotechnology
Publisher:Frontiers Media
Place of publication:Lausanne
Document Type:Article
Language:English
Year of Publication:2020
Volume:8
Page Number:11
First Page:1
Last Page:11
Article Number:388
DDC classes:570 Biowissenschaften, Biologie
Open Access?:Ja
Licence (German):License Logo  Creative Commons - CC BY - Namensnennung 4.0 International