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Bone remodeling can be mimicked in vitro by co-culture models. Based on bone cells, such co-cultures help to study synergistic morphological changes and the impact of materials and applied substances. Hence, we examined the formation of osteoclasts on bovine bone materials to prove the bone resorption functionality of the osteoclasts in three different co-culture set-ups using human monocytes (hMCs) and (I) human mesenchymal stem cells (hMSCs), (II) osteogenic differentiated hMSCs (hOBs), and (III) hOBs in addition of soluble monocyte-colony stimulating factor (M CSF) and cytokine receptor activator of NFkB ligand (RANKL).We detected osteoclast-specific actin morphology, as well as the expression of cathepsin K and CD51/61 in single cells in set-up II and in numerous cells in set-up III. Resorption pits on bone material as characteristic proof of functional osteoclasts were not found in set-up I and II, but we detected such resorption pits in set–up III. We conclude in co culture models without M-CSF and RANKL that monocytes can differentiate into osteoclasts that show the characteristic actin structures and protein expression. However, to receive functional bone resorbing osteoclasts in vitro, the addition of M-CSF and RANKL is needed. Moreover, we suggest the use of bone or bone-like materials for future studies evaluating osteoclastogenesis.
To date, special interest has been paid to composite scaffolds based on polymers enriched with hydroxyapatite (HA). However, the role of HA containing different trace elements such as silicate in the structure of a polymer scaffold has not yet been fully explored. Here, we report the potential use of silicate-containing hydroxyapatite (SiHA) microparticles and microparticle aggregates in the predominant range from 2.23 to 12.40 μm in combination with polycaprolactone (PCL) as a hybrid scaffold with randomly oriented and well-aligned microfibers for regeneration of bone tissue. Chemical and mechanical properties of the developed 3D scaffolds were investigated with XRD, FTIR, EDX and tensile testing. Furthermore, the internal structure and surface morphology of the scaffolds were analyzed using synchrotron X-ray μCT and SEM. Upon culturing human mesenchymal stem cells (hMSC) on PCL-SiHA scaffolds, we found that both SiHA inclusion and microfiber orientation affected cell adhesion. The best hMSCs viability was revealed at 10 day for the PCL-SiHA scaffolds with well-aligned structure (~82%). It is expected that novel hybrid scaffolds of PCL will improve tissue ingrowth in vivo due to hydrophilic SiHA microparticles in combination with randomly oriented and well-aligned PCL microfibers, which mimic the structure of extracellular matrix of bone tissue.
Lithographical hotspot (LH) detection using deep learning (DL) has received much attention in the recent years. It happens mainly due to the facts the DL approach leads to a better accuracy over the traditional, state-of-the-art programming approaches. The purpose of ths study is to compare existing data augmentation (DA) techniques for the integrated circuit (IC) mask data using DL methods. DA is a method which refers to the process of creating new samples similar to the training set, thereby helping to reduce the gap between classes as well as improving the performance of the DL system. Experimental results suggest that the DA methods increase overall DL models performance for the hotspot detection tasks.
Completely defined co-culture of adipogenic differentiated ASCs and microvascular endothelial cells
(2018)
Vascularized adipose tissue models are in high demand as alternatives to animal models to elucidate the mechanisms of widespread diseases, screen for new drugs or assess drug safety levels. Animal-derived sera such as fetal bovine serum (FBS), which are commonly used in these models, are associated with ethical concerns, risk of contaminations and inconsistencies of their composition and impact on cells. In this study, we developed a serum-free, defined co culture medium and implemented it in an adipocyte/endothelial cell (EC) co culture model.
Human adipose-derived stem cells were differentiated under defined conditions (diffASCs) and, like human microvascular ECs (mvECs), cultured in a defined co culture medium in mono-, indirect or direct co-culture for 14 days. The defined co-culture medium was superior when compared to mono-culture media and facilitated the functional maintenance and maturation of diffASCs including perilipin A expression, lipid accumulation, and also glycerol and leptin release. The medium also allowed mvEC maintenance, confirmed by the expression of CD31 and von Willebrand factor (vWF), and by acetylated low density lipoprotein (acLDL) uptake. Thereby, mvECs showed strong dependence on EC-specific factors. Additionally, mvECs formed vascular structures in direct co-culture with diffASCs.
The completely defined co-culture system allows for the serum-free culture of adipocyte/EC co-cultures and thereby represents a valuable and ethically acceptable tool for the culture and study of vascularized adipose tissue models.
This paper introduces a highly scalable heteromodular origami art technique for constructing 3D framework structures using elementary struts and connectors folded from uncut sheets of standard A4 office paper. The presented technique, named ZEBRA, allows the design of meter-scale architectural objects, such as truss bridges and towers, which are capable of bearing substantial mechanical loads. Moving parts, ranging from simple levers to complete multi-bar linkages, can be integrated into static frameworks using a set of kinematic extensions. An overview is given of how the ZEBRA system can be used to teach university students various theoretical and practical aspects of the engineering sciences in an entertaining and hands-on way.
Rattern unerwünscht
(2018)
Die Mesago Messe Frankfurt als Veranstalter der Formnext 2018 haben von Prof. Dr.-Ing. Steffen Ritter von der Hochschule Reutlingen eine anschauliche Übersicht erstellen lassen, welche die Vielfalt der unterschiedlichen Verfahren zur additiven Fertigung verständlich erklärt. Die kleine, zweisprachige Broschüre mit dem Titel „AM Field Guide“ bietet auf 12 Seiten strukturierte Vergleichsdarstellungen von insgesamt 19 gängigen 3-D-Druck Technologien für Kunststoffe, Metalle sowie andere Werkstoffe und dazu noch einen Überblick über die Phasen und Prozessschritte in Projekten für Bauteile, die additiv hergestellt werden.
Nowadays robust, energy-efficient multisensor microsystems often come with heavily restricted power budgets and the characteristic of remaining in certain states for a longer period of time. During this time frame there is no continuous clock signal required which gives the opportunity to suspend the clock until a new transition is requested. In this paper, we present a new topology for on-demand locally clocked finite state machines. The architecture combines a local adaptive clocking approach with synchronous and asynchronous components forming a quasi synchronous system. Using adaptive and local clocking comes with the advantages of reducing the power consumption while saving design effort when no global clock tree is needed. Combining synchronous and asynchronous components is beneficial compared to previous fully asynchronous approaches concerning the design restrictions. The developed topology is verified by the implementation and simulation of a temperature-ADC sensor system realized in a 180 nm process.
Functionalised particles are highly requested in materials research, as they can be used as vital components in many advanced applications such as smart materials, functional coatings, drug carrier systems or adsorption materials. In this study, furan-functionalised melamine-formaldehyde (MF) particles were successfully prepared for the first time using an organic sol-gel process. Commercially available 2-Aminomethylfuran (AMF) and 2-Aminomethyl-5-methylfuran (AMMF) were used as modifying agents. In the isolated polymer particles, a melamine (M) to modifying agent ratio of M:AMF mol/mol 2.04:1 and M:AMMF ratio of mol/mol 1.25:1 was used. The obtained particles were isolated in various centrifugation and re-dispersion cycles and analysed using ATR-FT-IR, Raman and solid state 13C NMR spectroscopy, TGA, SEM and DSC measurements. Upon functionalisation the size of the MF particles increased (MF 1.59 µm, 27% CV (coefficient of variation); MF-AMF 2.56 µm, 25% CV; MF-AMMF 2.20 µm, 35% CV). DSC measurements showed that another type of exothermic residual reactivity besides condensation-based curing takes place with the furan-modified particles that is not related to the liberation of volatile compounds. The newly obtained particles are able to undergo Diels-Alder reactions with maleimide groups. The characteristic IR and Raman absorbance bands of the reaction products after the particles were reacted with 4,4′-Diphenylmethanebismaleimide reagent confirm the formation of a Diels-Alder adduct.
Impact of phenolic resin preparation on its properties and its penetration behavior in Kraft paper
(2018)
The core of decorative laminates is generally made of stacked Kraft paper sheets impregnated with a phenolic resin. As the impregnation process in industry is relatively fast, new methods need to be developed to characterize it for different paper-resin systems. Several phenolic resins were synthesized with the same Phenol:Formaldehyde ratio of 1:1.8 and characterized by Fourier Transform Infrared Spectrometry (FTIR) as well as Size-Exclusion Chromatography (SEC). In addition, their viscosities and surface tensions when diluted in methanol to 45% of solid content were measured. The capacity of each resin to penetrate a Kraft paper sheet was characterized using a new method, which measures the conductivities induced by the liquid resin crossing the paper substrate. With this method, crossing times could be measured with a good accuracy. Surprisingly, the results showed that the penetration time of the resin samples is not correlated to the viscosity values, but rather to the surface tension characteristics and the chemical characteristics of paper. Furthermore, some resins had a higher swelling effect on the fibers that delayed the crossing of the liquid through the paper.
An interactive clothing design and a personalized virtual display with user’s own face are presented in this paper to meet the requirement of personalized clothing customization. A customer interactive clothing design approach based on genetic engineering ideas is analyzed by taking suit as an example. Thus, customers could rearrange the clothing style elements, chose available color, fabric and come up with their own personalized suit style. A web 3D customization prototype system of personalized clothing is developed based on the Unity3D and VR technology. The layout of the structure and functions combined with the flow of the system are given. Practical issues such as 3D face scanning, suit style design, fabric selection, and accessory choices are addressed also. Tests to the prototype system indicate that it could show realistic clothing and fabric effect and offer effective visual and customization experience to users.
This paper investigates the evaluation of dense 3D face reconstruction from a single 2D image in the wild. To this end, we organise a competition that provides a new benchmark dataset that contains 2000 2D facial images of 135 subjects as well as their 3D ground truth face scans. In contrast to previous competitions or challenges, the aim of this new benchmark dataset is to evaluate the accuracy of a 3D dense face reconstruction algorithm using real, accurate and high-resolution 3D ground truth face scans. In addition to the dataset, we provide a standard protocol as well as a Python script for the evaluation. Last, we report the results obtained by three state-of-the-art 3D face reconstruction systems on the new benchmark dataset. The competition is organised along with the 2018 13th IEEE Conference on Automatic Face & Gesture Recognition.
Annotations of subject IDs in images are very important as ground truth for face recognition applications and news retrieval systems. Face naming is becoming a significant research topic in news image indexing applications. By exploiting the uniqueness of name, face naming is transformed to the problem of multiple instance learning (MIL) with exclusive constraint, namely the eMIL problem. First, the positive bags and the negative bags are automatically annotated by a hybrid recurrent convolutional neural network and a distributed affinity propagation cluster. Next, positive instance selection and updating are used to reduce the influence of false-positive bag and to improve the performance. Finally, max exclusive density and iterative Max-ED algorithms are proposed to solve the eMIL problem. The experimental results show that the proposed algorithms achieve a significant improvement over other algorithms.
Background aims: In vitro engineered adipose tissue is in great demand to treat lost or damaged soft tissue or to screen for new drugs, among other applications.However, today most attempts depend on the use of animal-derived sera. To pave the way for the application of adipose tissue-engineered
products in clinical trials or as reliable and robust in vitro test systems, sera should be completely excluded from the production process. In this study, we aimed to develop an in vitro adipose tissue model in the absence of sera and maintain its function long-term.
Methods: Human adipose tissue-derived stem cells were expanded and characterized in a xeno- and serum-free environment. Adipogenic differentiation was induced using a completely defined medium. Developed adipocytes were maintained in a completely defined maturation medium for additional 28 days. In addition to cell-viability and adherence, adipocyte-specific markers such as perilipin A expression of leptin release were evaluated.
Results: The defined differentiation medium enhanced cell adherence and lipid
accumulation at a significant level compared with the corresponding negative control. The defined maturation medium also significantly supported cell adherence and functional adipocyte maturation during the long-term culture period.
Conclusions: The process described here enables functional adipocyte generation and maintenance without the addition fo unknown or unimal-derived constituents, achieving an important milestone in the introduction of adipose tissue engineered products into clinical trials or in vitro screening.
The coculture of osteogenic and angiogenic cells and the resulting paracrine signaling via soluble factors are supposed to be crucial for successfully engineering vascularized bone tissue equivalents. In this study, a coculture system combining primary human adiposederived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryloyl‐modified gelatin (GM) as three‐dimensional scaffolds was examined for its support of tissue specific cell functions. HDMECs, together with hASCs as supporting cells, were encapsulated in soft GM gels and were indirectly cocultured with hASCs encapsulated in stiffer GM hydrogels additionally containing methacrylate‐modified hyaluronic acid and hydroxyapatite particles. After 14 days, the hASC in the stiffer gels (constituting the “bone gels”) expressed matrix proteins like collagen type I and fibronectin, as well as bone‐specific proteins osteopontin and alkaline phosphatase. After 14 days of coculture with HDMEC‐laden hydrogels, the viscoelastic properties of the bone gels were significantly higher compared with the gels in monoculture. Within the soft vascularization gels, the formed capillary‐like networks were significantly longer after 14 days of coculture than the structures in the control gels. In addition, the stability as well as the complexity of the vascular networks was significantly increased by coculture. We discussed and concluded that osteogenic and angiogenic signals from the culture media as well as from cocultured cell types, and tissue‐specific hydrogel composition all contribute to stimulate the interplay between osteogenesis and angiogenesis in vitro and are a basis for engineering vascularized bone.
Early reduction of risks in a startup or an innovation project is highly important. Appropriate means for risk reduction, such as testing business models with different kinds of experiments exist. However, deciding what to test and how to select the right test, is challenging for many startups and innovation projects. This article presents the so-called Business Experiments Navigator (BEN), a toolkit to assist startup and innovation processes. It compliments other tools such as the Business Model Canvas or the Lean Startup process. The main contribution of BEN is to bridge the gap between the riskiest assumptions of a business model and the multitude of available testing techniques by providing assumption templates. The Business Experiments Navigator has been validated in several workshops. Results show that it creates awareness among the workshop participants that a business model is based on assumptions which impose risks and need to be validated. Further, users of BEN were able to identify relevant assumptions and map different kinds of assumptions to appropriate testing techniques. The process applied in the workshops, as well as the assumption templates, helped the participants understand the main concepts and transfer their learnings, to their own business ideas.
Fitting 3D Morphable Face Models (3DMM) to a 2D face image allows the separation of face shape from skin texture, as well as correction for face expression. However, the recovered 3D face representation is not readily amenable to processing by convolutional neural networks (CNN). We propose a conformal mapping from a 3D mesh to a 2D image, which makes these machine learning tools accessible by 3D face data. Experiments with a CNN based face recognition system designed using the proposed representation have been carried out to validate the advocated approach. The results obtained on standard benchmarking data sets show its promise.
Research and Development (R&D) is crucial for the growth and future success of research-based pharma companies. To maintain their R&D organisations efficient, pharmaceutical companies started to hedge the potential of open innovation to cut R&D costs and to access external knowledge. These new strategies could be divided into several categories: open source, innovation centres, crowd sourcing and virtual R&D.
Die Überlebensfähigkeit von Unternehmen am Markt wird vorrangig durch den Input ihrer Lieferanten getrieben, da dem Einkauf bis zu 50% des prognostizierten Innovationspotenzials zugesprochen wird. Dabei gilt vor allem die digitale Transformation als wesentliche Voraussetzung, um die eigene Wettbewerbsfähigkeit langfristig zu sichern. Der vorliegende Beitrag setzt sich daher mit dem Digitalisierungspotenzial strategischer Lieferantenbeziehungen auseinander. Aufbauend auf einem konzeptionellen Modell des Einkaufs 4.0 werden dessen Erfolgstreiber analysiert. Neben der Optimierung von Einkaufsprozessen durch eProcurementlösungen sollte der Fokus auf der Beziehungspflege zu Lieferanten liegen, um das Potenzial von Einkauf 4.0 vollständig ausschöpfen zu können. Denn nur durch den Aufbau strategischer Wertschöpfungspartnerschaften lässt sich das Erfolgspotenzial des Einkaufs langfristig heben.