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Many start-ups are in search of cooperation partners to develop their innovative business models. In response, incumbent firms are introducing increasingly more cooperation systems to engage with start-ups. However, many of these cooperations end in failure. Although qualitative studies on cooperation models have tried to improve the effectiveness of incumbent start-up strategies, only a few have empirically examined start-up cooperation behavior. Considering the lack of adequate measurement models in current research, this paper focuses on developing a multi-item scale on cooperation behavior of start-ups, drawing from a series of qualitative and quantitative studies. The resultant scale contributes to recent research on start-up cooperation and provides a framework to add an empirical perspective to current research.