570 Biowissenschaften, Biologie
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We present an approach for segmenting individual cells and lamellipodia in epithelial cell clusters using fully convolutional neural networks. The method will set the basis for measuring cell cluster dynamics and expansion to improve the investigation of collective cell migration phenomena. The fully learning-based front-end avoids classical feature engineering, yet the network architecture needs to be designed carefully. Our network predicts how likely each pixel belongs to one of the classes and, thus, is able to segment the image. Besides characterizing segmentation performance, we discuss how the network will be further employed.
Rapid prototyping platforms reduce development time by allowing quick prototyping of a prototype idea and achieve more time for actual application development with user interfaces. This approach has long been followed in technical platforms, such as the Arduino. To transfer this form of prototyping to wearables, WearIT is presented in this paper.WearIT consists of four components as a wearable prototyping platform: (1) a vest, (2) sensor and actuator shields, (3) its own library and (4) a motherboard consisting of Arduino, Raspberry Pi, a board and a GPS module. As a result, a wearable prototype can be quickly developed by attaching sensor and actuator shields to the WearIT vest. These sensor and actuator shields can then be programmed through the WearIT library. Via Virtual Network Computing (VNC) with a remote computer, the screen contents of the Raspberry Pi can be accessed and the Arduino be programmed.