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Balancing purification and ultrastructure of naturally derived bone blocks for bone regeneration: report of the purification effort of two bone blocks

  • The present publication reports the purification effort of two natural bone blocks, that is, an allogeneic bone block (maxgraft®, botiss biomaterials GmbH, Zossen, Germany) and a xenogeneic block (SMARTBONE®, IBI S.A., Mezzovico Vira, Switzerland) in addition to previously published results based on histology. Furthermore, specialized scanning electron microscopy (SEM) and in vitro analyses (XTT, BrdU, LDH) for testing of the cytocompatibility based on ISO 10993-5/-12 have been conducted. The microscopic analyses showed that both bone blocks possess a trabecular structure with a lamellar subarrangement. In the case of the xenogeneic bone block, only minor remnants of collagenous structures were found, while in contrast high amounts of collagen were found associated with the allogeneic bone matrix. Furthermore, only island-like remnants of the polymer coating in case of the xenogeneic bone substitute seemed to be detectable. Finally, no remaining cells or cellular remnants were found in both bone blocks. The in vitro analyses showed that both bone blocks are biocompatible. Altogether, the purification level of both bone blocks seems to be favorable for bone tissue regeneration without the risk for inflammatory responses or graft rejection. Moreover, the analysis of the maxgraft® bone block showed that the underlying purification process allows for preserving not only the calcified bone matrix but also high amounts of the intertrabecular collagen matrix.

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Metadaten
Name:Krastev, Rumen
URN:urn:nbn:de:bsz:rt2-opus4-25477
DOI:https://doi.org/10.3390/ma12193234
ISSN:1996-1944
Erschienen in:Materials / Molecular Diversity Preservation International
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Year of Publication:2019
Tag:allogeneic; bone block; bone regeneration; dentistry; purification; xenogeneic
Volume:12
Issue:19, Aufsatz 3234
Pagenumber:13
First Page:1
Last Page:13
Dewey Decimal Classification:600 Technik, Medizin, angewandte Wissenschaften
Open Access:Ja
Licence (German):License Logo  Creative Commons - CC BY - Namensnennung 4.0 International