A novel LED-based 2D-fluorescence spectroscopy system for in-line monitoring of Chinese hamster ovary cell cultivations - Part I
- A new two-dimensional fluorescence sensor system was developed for in-line monitoring of mammalian cell cultures. Fluorescence spectroscopy allows for the detection and quantification of naturally occurring intra- and extracellular fluorophores in the cell broth. The fluorescence signals correlate the the cells' current redox state and other relevant process parameters. Cell culture pretests with twelve different excitation wavelengths showed that only three wavelengths account for a vast majority of spectral variation. Accordingly, the newly developed device utilizes three high-power LEDs as excitation sources in combination with a back-thinned CCD-spectrometer for fluorescence detection.
| Author of HS Reutlingen | Rebner, Karsten |
|---|---|
| URN: | urn:nbn:de:bsz:rt2-opus4-24088 |
| DOI: | https://doi.org/10.1002/elsc.201800149 |
| ISSN: | 1618-0240 |
| eISSN: | 1618-2863 |
| Erschienen in: | Engineering in life sciences |
| Publisher: | Wiley-VCH |
| Place of publication: | Weinheim |
| Document Type: | Article |
| Language: | English |
| Year of Publication: | 2019 |
| Tag: | 2D-fluorescence spectroscopy; CHO cell cultivation; MVDA; PAT; in-line biprocess monitoring; metabolism monitoring |
| Volume: | 19 |
| Issue: | 5 |
| Page Number: | 11 |
| First Page: | 352 |
| Last Page: | 362 |
| DDC classes: | 660 Technische Chemie |
| Open Access?: | Ja |
| Licence (German): |  Creative Commons - CC BY - Namensnennung 4.0 International |
