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We report an investigation into the distribution of copper oxidation states in oxide films formed on the surfaces of technical copper. The oxide films were grown by thermal annealing at ambient conditions and studied using Auger depth profiling and UV–Vis spectroscopy. Both Auger and UV–Vis data were evaluated applying multivariate curve resolution (MCR). Both experimental techniques revealed that the growth of Cu2O dominates the initial ca. 40 nm of oxide films grown at 175 °C, while further oxide growth is dominated by CuO formation. The largely coincident results from both experimental approaches demonstrates the huge benefit of the application of UV–Vis spectroscopy in combination with MCR analysis, which provides access to information on chemical state distributions without the need for destructive sample analysis. Both approaches are discussed in detail.
Homogeneous and monodispersed furan functionalised melamine-formaldehyde particles were produced. As a precursor, 2-chloro-1,3,5-triazine-2,4-diamine (Mel) was selectively substituted with 2-aminomethyl furan (Fu) units in a convenient one step reaction. The pure reaction product Fu-Mel, which was used without further purification, was reacted with formaldehyde by conventional sol-gel condensation in aqueous medium to yield chemically homogenous, spherically shaped and monodispersed particles. The particles were analysed using ATR-FT-IR, Raman, 1H and 13C NMR spectroscopy, TGA, SEM and DSC measurements. The reactivity of the furan groups located at the particle surface was studied by performing a thermoreversible Diels-Alder cycloaddition reaction with bis-maleimide coupling agents. The formed networks showed thermoreversible behaviour, which was characterised by dynamic IR and DSC measurements.
In der zunehmenden Individualisierung von Produkten zeigt sich, dass Kundennähe und digital vernetzte Zusammenarbeit aller Partner wertvolle Erfolgspotenziale darstellen. Für komplexe Kundenauftragsprozesse gilt es, zu vernetzen und die Prozesse und Systeme in Form eines ganzheitlichen Ansatzes zukunftsfähig zu gestalten. Dabei wird der Herausforderung begegnet, Daten und Dokumente zu digitalisieren und den manuellen Aufwand zu reduzieren. Der Untersuchungsgegenstand ist der Abwicklungsprozess, ausgehend von einer Online-Konfiguration durch den Kunden bis zur Bestellabwicklung. In diesem Beitrag wird ein Vorgehensmodell aufgezeigt, das Unternehmen in die Lage versetzt, ihren Kundenauftragsprozess durch ein digitales Geschäftsmodell zukunftsfähig auszugestalten. Nutzenpotenziale sind eine verstärkte Kundenbindung durch eng verzahnte digitale Kollaboration, verstärkte Wirtschaftlichkeit durch Reduktion der Prozesskosten sowie eine Optimierung der Customer Experience durch effiziente Abläufe.
A new two-dimensional fluorescence sensor system was developed for in-line monitoring of mammalian cell cultures. Fluorescence spectroscopy allows for the detection and quantification of naturally occurring intra- and extracellular fluorophores in the cell broth. The fluorescence signals correlate the the cells' current redox state and other relevant process parameters. Cell culture pretests with twelve different excitation wavelengths showed that only three wavelengths account for a vast majority of spectral variation. Accordingly, the newly developed device utilizes three high-power LEDs as excitation sources in combination with a back-thinned CCD-spectrometer for fluorescence detection.
Die digitale Transformation verändert branchenübergreifend Geschäftsmodelle und ist damit ein Schlüssel für den langfristigen Unternehmenserfolg. In der Praxis scheitert sie jedoch häufig an der richtigen Umsetzung. Der Digital-Business-Excellence-Ansatz soll Unternehmen helfen, den digitalen Wandel erfolgreich zu gestalten.
Indoor localization systems are becoming more and more important with the digitalization of the industrial sector. Sensor data such as the current position of machines, transport vehicles, goods or tools represent an essential component of cyber physical production systems (CCPS). However, due to the high costs of these sensors, they are not widespread and are used mainly in special scenarios. However, especially optical indoor positioning systems (OIPS) based on cameras have certain advantages due to their technological specifications. In this paper, the application scenarios and requirements as well as their characteristics are presented and a classification approach of OIPS is introduced.
Stronger than they look
(2019)
An important shift in software delivery is the definition of a cloud service as an independently deployable unit by following the microservices architectural style. Container virtualization facilitates development and deployment by ensuring independence from the runtime environment. Thus, cloud services are built as container based systems - a set of containers that control the lifecycle of software and middleware components. However, using containers leads to a new paradigm for service development and operation: Self service environments enable software developers to deploy and operate container based systems on their own - you build it, you run it. Following this approach, more and more operational aspects are transferred towards the responsibility of software developers. In this work, we propose a concept for self-adaptive cloud services based on container virtualization in line with the microservices architectural style and present a model-based approach that assists software developers in building these services. Based on operational models specified by developers, the mechanisms required for self-adaptation are automatically generated. As a result, each container automatically adapts itself in a reactive, decentralized manner. We evaluate a prototype which leverages the emerging TOSCA standard to specify operational behavior in a portable manner.
In vitro models of human adipose tissue may serve as beneficial alternatives to animal models to study basic biological processes, identify new drug targets, and as soft tissue implants. With this approach, we aimed to evaluate adipose-derived stem cells (ASC) and mature adipocytes (MA) comparatively for the application in the in vitro setup of adipose tissue constructs to imitate native adipose tissue physiology. We used human primary MAs and human ASCs, differentiated for 14 days, and encapsulated them in collagen type I hydrogels to build up a three-dimensional (3D) adipose tissue model. The maintenance of the models was analyzed after seven days based on a viability staining. Further, the expression of the adipocyte specific protein perilipin A and the release of leptin and glycerol were evaluated. Gene transcription profiles of models based on dASCs and MAs were analyzed with regard to native adipose tissue. Compared to MAs, dASCs showed an immature differentiation state. Further, gene transcription of MAs suggests a behavior closer to native tissue in terms of angiogenesis, which supports MAs as preferred cell type. In contrast to native adipose tissue, genes of de novo lipogenesis and tissue remodeling were upregulated in the in vitro attempts.
