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After considering significant literature on sustainable supply chain management (SSCM), it is evident that research has neglected the social dimension and still lacks in highlighting the role of sourcing intermediaries in supply chains. The apparel supply chain has increased enormously in length and complexity, driving apparel retailers to employ sourcing intermediaries who manage their sourcing activities with suppliers from developing countries overseas. Thus, the purpose of this study is to enrich existing findings on SSCM by exploring the management of social sustainability when sourcing intermediaries are in between the focal company and the respective developing country factories. More specifically, this study aims to understand the role of apparel sourcing intermediaries for the implementation of social management strategies based on the perception of multiple supply chain actors. Qualitative data was collected through semi-structured interviews conducted in Vietnam and Europe. Ultimately ten propositions are presented, all explicitly concentrating on the apparel intermediary’s role as a significant enabler for social sustainability in apparel supply chains. The roles are social sustainability, supplier developer and coordinator, gatekeeper and safeguard, cultural broker, and social risk manager. The social sustainability roles assumed by the apparel sourcing intermediary offer great opportunities to both apparel retailers and developing country factories.
Strategy to test mobile apps
(2014)
Nowadays the development of a mobile app implies challenges and difficulties, which have to be faced by mobile app developers. Innovations lead to a rapidly evolving mobile app market, therefore apps should be developed faster and offered in short release cycles to the market. Testing is a decisive activity within the development process that helps to improve the quality of the app. This research paper describes a strategy to test mobile apps that overcomes the challenges that mobile apps confront and permits to test the app in a structural test environment.
Motor-based theories of facial expression recognition propose that the visual perception of facial expression is aided by sensorimotor processes that are also used for the production of the same expression. Accordingly, sensorimotor and visual processes should provide congruent emotional information about a facial expression. Here, we report evidence that challenges this view. Specifically, the repeated execution of facial expressions has the opposite effect on the recognition of a subsequent facial expression than the repeated viewing of facial expressions. Moreover, the findings of the motor condition, but not of the visual condition, were correlated with a nonsensory condition in which participants imagined an emotional situation. These results can be well accounted for by the idea that facial expression recognition is not always mediated by motor processes but can also be recognized on visual information alone.
A fully passive RFID temperature sensor SoC with an accuracy of ±0.4°C (3σ) from 0°C to 125°C
(2018)
This paper presents a fully passive 13.56 MHz RFID temperature sensor system-on-chip. Its power management unit (PMU) operates over a large temperature range using a zero temperature coefficient (TC) bias source. On-chip temperature sensing is accomplished with low voltage, low power CMOS circuitry and time-domain signal processing. Two operating modes have been defined to study supply noise sensitivity: command mode and listening mode, which represent sensor operation during RFID command transfer and listening, respectively. Besides a standard readout command, a customized serial readout command is utilized to distinguish the data from both modes. In command mode, the sensor suffers from interference from the RFID command packet and outputs interference as well, while the sensor outputs no interference in listening mode. Measurements show that sensor resolution in listening mode is improved by a factor of approximately 16 compared to command mode. The chip was fabricated in a standard 0.35 µm CMOS technology and chip-on-board mounted to a tuned RFID transponder coil on an aluminium core FRA4 PCB substrate. Real-time wireless temperature sensing has been demonstrated with a commercial HF RFID reader. With a two-point calibration, the SoC achiesves a 3σ sensing accuracy of ±0.4°C from 0° C to 125° C.
Micro grids often consist of energy generators, storages and consumers with controllers which are not prepared for their integration into communication networks for energy systems. In this paper it will be presented, how standards from the field of energy automation can be applied in such controllers. The data for communication interfaces can be structured according to the IEC 61850- or the VHPREADY standard. It is investigated which requirements must be supported to implement such data models within the controllers. For the transmission of the data we propose the OPC UA protocol, which supports extensive security measures and which is today available for nearly all modern types of controllers and computers.
For autonomously driving cars and intelligent vehicles it is crucial to understand the scene context including objects in the surrounding. A fundamental technique accomplishing this is scene labeling. That is, assigning a semantic class to each pixel in a scene image. This task is commonly tackled quite well by fully convolutional neural networks (FCN). Crucial factors are a small model size and a low execution time. This work presents the first method that exploits depth cues together with confidence estimates in a CNN. To this end, novel experimentally grounded network architecture is proposed to perform robust scene labeling that does not require costly preprocessing like CRFs or LSTMs as commonly used in related work. The effectiveness of this approach is demonstrated in an extensive evaluation on a challenging real-world dataset. The new architecture is highly optimized for high accuracy and low execution time.
Turbidity sensing is very common in the control of drinking water. Furthermore, turbidity measurements are applied in the chemical (e.g., process monitoring), pharmaceutical (e.g., drug discovery), and food industries (e.g., the filtration of wine and beer). The most common measurement technique is nephelometric turbidimetry. A nephelometer is a device for measuring the amount of scattered light of suspended particles in a liquid by using a light source and a light detector orientated in 90°to each other. Commercially available nephelometers cost usually—depending on the measurable range, reliability, and precision —thousands of euros. In contrast, our new developed GRIN-lens-based nephelometer, called GRINephy, combines low costs with excellent reproducibility and precision, even at very low turbidity levels, which is achieved by its ability to rotate the sample. Thereby, many cuvette positions can be measured, which results in a more precise average value for the turbidity calculated by an algorithm, which also eliminates errors caused by scratches and contaminations on the cuvettes. With our compact and cheap Arduino-based sensor, we are able to measure in the range of 0.1–1000 NTU and confirm the ISO 7027-1:2016 for low turbidity values.
There are several intra-operative use cases which require the surgeon to interact with medical devices. I used the Leap Motion Controller as input device for three use-cases: 2D-interaction (e.g. advancing EPR data), selection of a value (e.g. room illumination brightness) and an application point and click scenario. I evaluated the Palm Mouse as the most suitable gesture solution to coordinate the mouse and advise to use the implementation using all fingers to perform a click. This small case study introduces the implementations and methods that result those recommendations.
Reconstructing 3D face shape from a single 2D photograph as well as from video is an inherently ill-posed problem with many ambiguities. One way to solve some of the ambiguities is using a 3D face model to aid the task. 3D morphable face models (3DMMs) are amongst the state of the art methods for 3D face reconstruction, or so called 3D model fitting. However, current existing methods have severe limitations, and most of them have not been trialled on in-the-wild data. Current analysis-by- synthesis methods form complex non linear optimisation processes, and optimisers often get stuck in local optima. Further, most existing methods are slow, requiring in the order of minutes to process one photograph.
This thesis presents an algorithm to reconstruct 3D face shape from a single image as well as from sets of images or video frames in real-time. We introduce a solution for linear fitting of a PCA shape identity model and expression blendshapes to 2D facial landmarks. To improve the accuracy of the shape, a fast face contour fitting algorithm is introduced. These different components of the algorithm are run in iteration, resulting in a fast, linear shape-to- landmarks fitting algorithm. The algorithm, specifically designed to fit to landmarks obtained from in-the-wild images, by tackling imaging conditions that occur in in-the-wild images like facial expressions and the mismatch of 2D–3D contour correspondences, achieves the shape reconstruction accuracy of much more complex, nonlinear state of the art methods, while being multiple orders of magnitudes faster.
Second, we address the problem of fitting to sets of multiple images of the same person, as well as monocular video sequences. We extend the proposed shape-to-landmarks fitting to multiple frames by using the knowledge that all images are from the same identity. To recover facial texture, the approach uses texture from the original images, instead of employing the often-used PCA albedo model of a 3DMM. We employ an algorithm that merges texture from multiple frames in real-time based on a weighting of each triangle of the reconstructed shape mesh.
Last, we make the proposed real-time 3D morphable face model fitting algorithm available as open-source software. In contrast to ubiquitous available 2D-based face models and code, there is a general lack of software for 3D morphable face model fitting, hindering a widespread adoption. The library thus constitutes a significant contribution to the community.
Thematic issue on human-centred ambient intelligence: cognitive approaches, reasoning and learning
(2017)
This editorial presents advances on human-centred Ambient Intelligence applications which take into account cognitive issues when modelling users (i.e. stress, attention disorders), and learn users’ activities/preferences and adapt to them (i.e. at home, driving a car). These papers also show AmI applications in health and education, which make them even more valuable for the general society.
The main challenge when driving heat pumps by PV-electricity is balancing differing electrical and thermal demands. In this article, a heuristic method for optimal operation of a heat pump driven by a maximum share of PV-electricity is presented. For this purpose, the (DHW) are activated in order shift the operation of the heat pump to times of PV-generation. The system under consideration refers to thermal and electrical demands of a single family house. It consists of a heat pump, a thermal energy storage for DHW and of grid connected heating and generation of domestic hot water, the heat pump runs with two different supply temperatures and thereby achieving a maximum overall COP. Within the algorithm for optimization a set of heuristic rules is developed in a way that the operational characteristics of the heat pump in terms of minimum running and stopping times are met as well as the limiting constraints of upper and lower limits of room temperature and energy content of electricity generated, a varying number of heat pump schedules fulfilling the bundary conditions are created. Finally, the schedule offering the maximum on-site utilization of PV-electricity with a minimum number of starts of the heat pump, which serves as secondary condition, is selected. Yearly simulations of this combination have been carried out. Initial results of this method indicate a significant rise in on-site consumption of the PV-electricity and heating demand fulfilment by renewable electricity with no need for a massive TES for the heating system in terms of a big water tank.
Consistent supply chain management across all levels of value creation is a common approach in the industrial sector. The implementation in agricultural processes requires rethinking in the supply chain concept. The reasons are the heuristic characterized processes, the stochastic environmental conditions, the mobility of the production facilities and the low division of work.
In this paper we deal with how concepts of innovative supply chain management of Industrie 4.0 could not only deliver a way to overcome said problems but also provide the foundation for the development of new forms of work and business models for Farming 4.0.
Relocation of production to countries with low labour costs has induced increased labour market flexibility, which has been praised as a silver bullet for economic growth and low unemployment. Within a unionised oligopoly framework, in which a multinational firm has the option to relocate its production to a foreign country, we analyse the welfare implications of both centralised and flexible wage-setting regimes. For very low foreign wages, wage flexibility leads to higher welfare than a rigid centralised regime. In contrast, for ‘intermediate’ wage levels in the foreign country, an industry-wide uniform wage leads to higher social welfare than flexible wages.
One of strategically important issues of energy security of Ukraine and the countries of Europe today is to reduce the consumption of natural gas. This task is particularly relevant in winter, when a significant amount of natural gas is consumed for heating premises. Therefore, one can predict that in the nearest future, in Ukraine and European countries, premises will be heated more frequently by electrical energy.
A massive transition to electric heating of premises under conditions of the implementation of national objective in Ukraine and the countries of Europe related to a significant reduction in energy consumption necessitates to rethink the process of control over electric heating of premises. It is required that the algorithms that control power supply to premises should include mechanisms for planning the amount of electric energy consumed by an individual. This is especially true of such energy-intensive processes like heating the premises.
Therefore, it is an important task for Ukraine and the countries of Europe to work out an approach for creating systems to control electric heating of premises in a house or apartment that would take into consideration not only information about the desired temperature regime, but also information on the desired amount of electricity needed for heating.
This publication is a Technical report by the Joint Research Centre (JRC), the European Commission’s science and knowledge service. It aims to provide evidence-based scientific support to the European policymaking process.
This report provides an bird’s-eye view on the concept and implications of digital identities. After an introduction situating the concept of identity, the report clarifies its contemporary meaning and proposes a definition of reference. Subsequently, the authors examine the consequences of the translation of the concept of identity into the digital, internet connected world. They then analyse the particularities and consequences of this translation, which allows them to situate and define the concept of digital identities.
Finally, they conclude with the challenges that digital identity poses to the digital citizen in the attempt to manage and protect its attributes with the advent of Internet of Things and blockchain technology.
Painting galleries typically provide a wealth of data composed of several data types. Those multivariate data are too complex for laymen like museum visitors to first, get an overview about all paintings and to look for specific categories. Finally, the goal is to guide the visitor to a specific painting that he wishes to have a more closer look on. In this paper we describe an interactive visualization tool that first provides such an overview and lets people experiment with the more than 41,000 paintings collected in the web gallery of art. To generate such an interactive tool, our technique is composed of different steps like data handling, algorithmic transformations, visualizations, interactions, and the human user working with the tool with the goal to detect insights in the provided data. We illustrate the usefulness of the visualization tool by applying it to such characteristic data and show how one can get from an overview about all paintings to specific paintings.
How to protect the skin from getting sun burnt? The sun can damage your skin e.g. skin cancer. But the sun has a positive effect to the human. The time in sun and the intensity are key values between enjoy the sunbath and having a negative effect to the skin. A smart device like a UV flower could help you to enjoy the sunbath. It measures the UV index around you and gives this information to a smartphone app. The development steps of such a device are described in this paper. The UV flower is made of textile fabrics.
Medical applications are becoming increasingly important in the current development of health care and therefore a crucial part of the medical industry. An essential component is the development of user interfaces for mobile medical applications. The conceptual process is crucial for the further development of the main development process. Inconsistency or errors in the conceptual phase, have a serious impact on all areas and could prevent the certification for market approval.
This paper presents a guide to support developer with this process. It was developed based on a requirement analysis of the legal requirements to publish a medical device.
A sleep study is a test used to diagnose sleep disorders and is usually done in sleep laboratories. The golden standard for evaluation of sleep is overnight polysomnography (PSG). Unfortunately, in-lab sleep studies are expensive and complex procedures. Furthermore, with a minimum of 22 wire attachments to the patient for sleep recording, this medical procedure is invasive and unfamiliar for the subjects. To solve this problem, low-cost home diagnostic systems, based on noninvasive recording methods requires further researches.
For this intention it is important to find suitable bio vital parameters for classifying sleep phases WAKE, REM, light sleep and deep sleep without any physical impairment at the same time. We decided to analyse body movement (BM), respiration rate (RR) and heart rate variability (HRV) from existing sleep recordings to develop an algorithm which is able to classify the sleep phases automatically. The preliminary results of this project show that BM, RR and HRV are suitable to identify WAKE, REM and NREM stage.
To analyze the humans’ sleep it is necessary as to identify the sleep stages, occurring during the sleep, their durations and sleep cycles. The gold standard procedure for this approach is polysomnography (PSG), which classify the sleep stages based on Rechtschaffen and Kales (R-K) method. This method aside the advantages as high accuracy has however some disadvantages, among others time-consuming and uncomfortable for the patient procedure. Therefore, the development of further methods for the sleep classification in addition to PSG is a promising topic for the investigation and this work has as its aim the presentation of possible ways and goals for this development.
Asymmetric read/write storage technologies such as Flash are becoming
a dominant trend in modern database systems. They introduce
hardware characteristics and properties which are fundamentally
different from those of traditional storage technologies such
as HDDs.
Multi-Versioning Database Management Systems (MV-DBMSs)
and Log-based Storage Managers (LbSMs) are concepts that can
effectively address the properties of these storage technologies but
are designed for the characteristics of legacy hardware. A critical
component of MV-DBMSs is the invalidation model: commonly,
transactional timestamps are assigned to the old and the new version,
resulting in two independent (physical) update operations.
Those entail multiple random writes as well as in-place updates,
sub-optimal for new storage technologies both in terms of performance
and endurance. Traditional page-append LbSM approaches
alleviate random writes and immediate in-place updates, hence reducing
the negative impact of Flash read/write asymmetry. Nevertheless,
they entail significant mapping overhead, leading to write
amplification.
In this work we present an approach called Snapshot Isolation
Append Storage Chains (SIAS-Chains) that employs a combination
of multi-versioning, append storage management in tuple granularity
and novel singly-linked (chain-like) version organization.
SIAS-Chains features: simplified buffer management, multi-version
indexing and introduces read/write optimizations to data placement
on modern storage media. SIAS-Chains algorithmically avoids
small in-place updates, caused by in-place invalidation and converts
them into appends. Every modification operation is executed
as an append and recently inserted tuple versions are co-located.
IT Governance (ITG) is crucial due to its significant impact on enabling innovation and enhancing firm performance. Hence, in the last decade ITG has become important in both academic and in practical research. Although several studies have investigated individual aspects of ITG success and its impact on single determinants, the causal relationship of how ITG promotes firm performance remains unclear. Thus, a more comprehensive understanding about the link between ITG and firm performance is needed. To address this gap, this research aims at understanding how ITG and firm performance are related. Therefore, we conducted a systematic literature review (1) to create an overview on how current research structures the link between ITG mechanisms and firm performance, (2) to uncover key constructs as potential mediators or moderators on the general link between ITG and performance, and (3) to set the basis for future studies on the ITG-firm performance relationship.
We were able to identify a set of specific capabilities corporations need to develop in order to enhance brand love. Furthermore, the effects of most dynamic capabilities on brand love have a strong correlation to the degree of customer orientation. Other results are relevant concerning the proposed moderation and mediation hypotheses. Firstly, the impact of customer orientation on brand love is varied under specific market conditions, supporting our central moderation hypothesis (β = .259, p = .001). To be precise, the impact of customer orientation is strongest in markets that have low competitive differentiation in products and services. Other control variables like age, gender, or market form (B2B versus B2C) lead to no significant heterogeneity in the data set. Finally, mediation analyses show no significant “direct effect” of the existing DC constructs on brand love, supporting the mediating role of customer orientation.
Royal Philip's goal was to use innovation to improve the lives of three billion people a year by 2025. To reach that goal, the company was shifting from selling medical products in a transactional manner to providing integrated healthcare solutions based on digital health technology ("HealthTech").
This shift required a dual transformation. On one hand, the company needed to transform how healthcare was conducted. Healthcare professionals would have to change the way they worked and reimbursement schemes needed to change to incentivize payers, providers, and patients in vastly different ways. On the other hand, Philips needed to redesign how it worked internally. The company componentized its business, introduced digital platforms, and co-created solutions with the various stakeholders of the healthcare industry.
In other words: Royal Philips was transforming itself in order to reinvent healthcare in the digital age.
In 2016, German car manufacturer the Audi Group (AUDI AG) was working on an expanding array of digital innovations. The goals of these innovations varied, and included strengthening customer- and employee-facing processes, digitally enhancing existing products, and developing new, potentially disruptive business models. Audi’s IT unit was critical to each of these efforts. Based on personal interviews with 11 IT- and non-IT executives at Audi, this case examines the different ways in which digitization can help to enhance and transform an organization’s processes, products, and business models. The case also highlights the challenges that arise as large companies “digitize.”
Recent digital technologies like the Internet of Things and Augmented Reality have brought IT into companies’ core products. What were previously purely physical products are becoming hybrid or digitized. Despite receiving a lot of recent attention, digitized products have only seen a slow uptake in businesses so far. In this paper, we study the challenges that keep companies from realizing the desired impacts of digitized products and the practices they employ to address these challenges. To do so, we looked at companies from a set of industries that are highly affected by digital transformation, but at the same time hesitant to move to a more digitized world: the creative industries. Based on a literature review and twelve interviews in creative industries, we developed a conceptual model that can serve as a basis for formulating testable hypotheses for further research in this area.
Electronic word-of-mouth (eWoM) communication has received a lot of attention from the academic community. As multiple research papers focus on specific facets of eWoM, there is a need to integrate current research results systematically. Thus, this paper presents a scientific literature analysis in order to determine the current state-of-the-art in the field of eWoM.
Historically, research and development (R&D) in the pharmaceutical sector has predominantly been an in-house activity. To enable investments for game changing late-stage assets and to enable better and less costly go/no-go decisions, most companies have employed a fail early paradigm through the implementation of clinical proof-of-concept organizations. To fuel their pipelines, some pioneers started to complement their internal R&D efforts through collaborations as early as the 1990s. In recent years, multiple extrinsic and intrinsic factors induced an opening for external sources of innovation and resulted in new models for open innovation, such as open sourcing, crowdsourcing, public–private partnerships, innovations centres, and the virtualization of R&D. Three factors seem to determine the breadth and depth regarding how companies approach external innovation: (1) the company’s legacy, (2) the company’s willingness and ability to take risks and (3) the company’s need to control IP and competitors. In addition, these factors often constitute the major hurdles to effectively leveraging external opportunities and assets. Conscious and differential choices of the R&D and business models for different companies and different divisions in the same company seem to best allow a company to fully exploit the potential of both internal and external innovations.
This paper examines the efficacy of social media systems in customer complaint handling. The emergence of social media, as a useful complement and (possibly) a viable alternative to the traditional channels of service delivery, motivates this research. The theoretical framework, developed from literature on social media and complaint handling, is tested against data collected from two different channels (hotline and social media) of a German telecommunication services provider, in order to gain insights into channel efficacy in complaint handling. We contribute to the understanding of firm’s technology usage for complaint handling in two ways:
(a) by conceptualizing and evaluating complaint handling quality across traditional and social media channels and (b) by comparing the impact of complaint handling quality on key performance outcomes such as customer loyalty, positive word-of-mouth, and crosspurchase intentions across traditional and social media channels.
Pokémon Go was the first mobile augmented reality (AR) game to reach the top of the download charts of mobile applications. However, little is known about this new generation of mobile online AR games. Existing theories provide limited applicability for user understanding. Against this background, this research provides a comprehensive framework based on uses and gratification theory, technology risk research, and flow theory. The proposed framework aims to explain the drivers of attitudinal and intentional reactions, such as continuance in gaming or willingness to invest money in in-app purchases. A survey among 642 Pokémon Go players provides insights into the psychological drivers of mobile AR games. The results show that hedonic, emotional, and social benefits and social norms drive consumer reactions while physical risks (but not data privacy risks) hinder consumer reactions. However, the importance of these drivers differs depending on the form of user behavior.
How to separate the wheat from the chaff: improved variable selection for new customer acquisition
(2017)
Steady customer losses create pressure for firms to acquire new accounts, a task that is both costly and risky. Lacking knowledge about their prospects, firms often use a large array of predictors obtained from list vendors, which in turn rapidly creates massive high-dimensional data problems. Selecting the appropriate variables and their functional relationships with acquisition probabilities is therefore a substantial challenge. This study proposes a Bayesian variable selection approach to optimally select targets for new customer acquisition. Data from an insurance company reveal that this approach outperforms nonselection methods and selection methods based on expert judgment as well as benchmarks based on principal component analysis and bootstrap aggregation of classification trees. Notably, the optimal results show that the Bayesian approach selects panel-based metrics as predictors, detects several nonlinear relationships, selects very large numbers of addresses, and generates profits. In a series of post hoc analyses, the authors consider prospects’ response behaviors and cross selling potential and systematically vary the number of predictors and the estimated profit per response. The results reveal that more predictors and higher response rates do not necessarily lead to higher profits.
Characterisation of porous knitted titanium for replacement of intervertebral disc nucleus pulposus
(2017)
Effective restoration of human intervertebral disc degeneration is challenged by numerous limitations of the currently available spinal fusion and arthroplasty treatment strategies. Consequently, use of artificial biomaterial implant is gaining attention as a potential therapeutic strategy. Our study is aimed at investigating and characterizing a novel knitted titanium (Ti6Al4V) implant for the replacement of nucleus pulposus to treat early stages of chronic intervertebral disc degeneration. Specific knitted geometry of the scaffold with a porosity of 67.67 ± 0.824% was used to overcome tissue integration failures. Furthermore, to improve the wear resistance without impairing original mechanical strength, electro-polishing step was employed. Electro-polishing treatment changed a surface roughness from 15.22 ± 3.28 to 4.35 ± 0.87 μm without affecting its wettability which remained at 81.03 ± 8.5°. Subsequently, cellular responses of human mesenchymal stem cells (SCP1 cell line) and human primary chondrocytes were investigated which showed positive responses in terms of adherence and viability. Surface wettability was further enhanced to super hydrophilic nature by oxygen plasma treatment, which eventually caused substantial increase in the proliferation of SCP1 cells and primary chondrocytes. Our study implies that owing to scaffolds physicochemical and biocompatible properties, it could improve the clinical performance of nucleus pulposus replacement.
A wide variety of cell types exhibit substrate topography-based behavior, also known as contact guidance. However, the precise cellular mechanisms underlying this process are still unknown. In this study, we investigated contact guidance by studying the reaction of human endothelial cells (ECs) to well-defined microgroove topographies, both during and after initial cell spreading. As the cytoskeleton plays a major role in cellular adaptation to topographical features, two methods were used to perturb cytoskeletal structures. Inhibition of actomyosin contractility with the chemical inhibitor blebbistatatin demonstrated that initial contact guidance events are independent of traction force generation. However, cell alignment to the grooved substrate was altered at later time points, suggesting an initial ‘passive’ phase of contact guidance, followed by a contractility-dependent ‘active’ phase that relies on mechanosensitive feedback. The actin cytoskeleton was also perturbed in an indirect manner by culturing cells upside down, resulting in decreased levels of contact guidance and suggesting that a possible loss of contact between the actin cytoskeleton and the substrate could lead to cytoskeleton impairment. The process of contact guidance at the microscale was found to be primarily lamellipodia driven, as no bias in filopodia extension was observed on micron-scale grooves.
Perivascular stromal cells, including mesenchymal stem/stromal cells (MSCs), secrete paracrine factor in response to exercise training that can facilitate improvements in muscle remodeling. This study was designed to test the capacity for muscle-resident MSCs (mMSCs) isolated from young mice to release regenerative proteins in response to mechanical strain in vitro, and subsequently determine the extent to which strain-stimulated mMSCs can enhance skeletal muscle and cognitive performance in a mouse model of uncomplicated aging. Protein arrays confirmed a robust increase in protein release at 24 h following an acute bout of mechanical strain in vitro (10%, 1 Hz, 5 h) compared to non-strain controls. Aged (24 month old), C57BL/6 mice were provided bilateral intramuscular injection of saline, non strain control mMSCs, or mMSCs subjected to a single bout of mechanical strain in vitro (4 ×104). No significant changes were observed in muscle weight, myofiber size, maximal force, or satellite cell quantity at 1 or 4 wks between groups. Peripheral perfusion was significantly increased in muscle at 4 wks post-mMSC injection (p < 0.05), yet no difference was noted between control and preconditioned mMSCs. Intramuscular injection of preconditioned mMSCs increased the number of new neurons and astrocytes in the dentate gyrus of the hippocampus compared to both control groups (p < 0.05), with a trend toward an increase in water maze performance noted (p=0.07). Results from this study demonstrate that acute injection of exogenously stimulated muscle-resident stromal cells do not robustly impact aged muscle structure and function, yet increase the survival of new neurons in the hippocampus.
Intermediate filament reorganization dynamically influences cancer cell alignment and migration
(2017)
The interactions between a cancer cell and its extracellular matrix (ECM) have been the focus of an increasing amount of investigation. The role of the intermediate filament keratin in cancer has also been coming into focus of late, but more research is needed to understand how this piece fits in the puzzle of cytoskeleton-mediated invasion and metastasis. In Panc-1 invasive pancreatic cancer cells, keratin phosphorylation in conjunction with actin inhibition was found to be sufficient to reduce cell area below either treatment alone. We then analyzed intersecting keratin and actin fibers in the cytoskeleton of cyclically stretched cells and found no directional correlation. The role of keratin organization in Panc-1 cellular morphological adaptation and directed migration was then analyzed by culturing cells on cyclically stretched polydimethylsiloxane (PDMS) substrates, nanoscale grates, and rigid pillars. In general, the reorganization of the keratin cytoskeleton allows the cell to become more ‘mobile’- exhibiting faster and more directed migration and orientation in response to external stimuli. By combining keratin network perturbation with a variety of physical ECM signals, we demonstrate the interconnected nature of the architecture inside the cell and the scaffolding outside of it, and highlight the key elements facilitating cancer cell-ECM interactions.
It has not yet been possible to achieve the desired aim of decoupling economic growth from global material demand. Small and medium sized enterprises (SMEs) represent the backbone of most industrialized economies. Although material efficiency is of vital importance for many SMEs, few of them actually treat it as their top priority. There is a cornucopia of tools and methods available, which can be used for material efficiency purposes. These, however, have gained little groud in the SME-field. This work deals with the enabling factors for material efficiency improvements in manufacturing SMEs and projections towards aspects of supply chain and circular economy. A multi-disciplinary decoupling approach for manufacturing SMEs and an implementation roadmap for further practical development are proposed. The approach combines appropriate complexity of technology and socio-economic considerations. It enables a connection to existing methods and the implementation of established information technologies.
AUDI AG has historically focused on producing and selling premium vehicles but has begun to experiment with providing mobility services, built around car sharing. Its response to the so-called sharing economy addressed strategic and transformational challenges. Strategically, the company pursued additional sources of revenue from targeted, premium mobility services, rather than the less segmented services provided by competitors such as BMW and Zipcar. AUDI AG also transformed its organizational structure, processes and architecture to balance autonomy for innovation and integration for competitiveness.
The maintenance issue of batteries and the limited power level of energy harvesting is addressed by the presented integrated micropower supply. Connected to the 120/230-VRMS mains, it provides a 3.3-V ac output voltage, suitable for applications such as the Internet-of Things and smart homes. The micropower supply consists of a fully integrated ac–dc and dc–dc converter with one external low-voltage surface mount device buffer capacitor, resulting in an extremely compact size. Fabricated in a low-cost 0.35-μm 700-V complimentary metal-oxide-semiconductor technology, it covers a die size of 7.7 mm². The ac–dc converter is a direct coupled, full-wave rectifier with a subsequent series regulator. The dc–dc stage is a fully integrated capacitive 4:1 converter with up to 17-V input and 47.4% peak efficiency. The power supply comprises several high-voltage control circuits including level shifters and various types of charge pumps (CPs). A source supplied CP is utilized that supports a varying switching node potential. The overall losses are discussed and optimized, including flying capacitor bottom-plate losses. The power supply achieves an output power of 3 mW, resulting in a power density of 390 μW/mm². This exceeds prior art by a factor of 11.
Cell-cell and cell-extracellular matrix (ECM) adhesion regulates fundamental cellular functions and is crucial for cell-material contact. Adhesion is influenced by many factors like affinity and specificity of the receptor-ligand interaction or overall ligand concentration and density. To investigate molecular details of cell ECM and cadherins (cell-cell) interaction in vascular cells functional nanostructured surfaces were used Ligand-functionalized gold nanoparticles (AuNPs) with 6-8 nm diameter, are precisely immobilized on a surface and separated by non-adhesive regions so that individual integrins or cadherins can specifically interact with the ligands on the AuNPs. Using 40 nm and 90 nm distances between the AuNPs and functionalized either with peptide motifs of the extracellular matrix (RGD or REDV) or vascular endothelial cadherins (VEC), the influence of distance and ligand specificity on spreading and adhesion of endothelial cells (ECs) and smooth muscle cells (SMCs) was investigated. We demonstrate that RGD-dependent adhesion of vascular cells is similar to other cell types and that the distance dependence for integrin binding to ECM-peptides is also valid for the REDV motif. VEC-ligands decrease adhesion significantly on the tested ligand distances. These results may be helpful for future improvements in vascular tissue engineering and for development of implant surfaces.
This work presents a fully integrated GaN gate driver in a 180nm HV BCD technology that utilizes high-voltage energy storing (HVES) in an on-chip resonant LC tank, without the need of any external capacitor. It delivers up to 11nC gate charge at a 5V GaN gate, which exceeds prior art by a factor of 45-83, supporting a broad range of GaN transistor types. The stacked LC tank covers an area of only 1.44mm², which corresponds to a superior value of 7.6nC/mm².
In recent years, significant progress was made on switched-capacitor DCDC converters as they enable fully integrated on chip power management. New converter topologies overcame the fixed input-to-output voltage limitation and achieved high efficiency at high power densities. SC converters are attractive to not only mobile handheld devices with small input and output voltages, but also for power conversion in IoTs, industrial and automotive applications, etc. Such applications need to be capable of handling high input voltages of more than 10V. This talk highlights the challenges of the required supporting circuits and high voltage techniques, which arise for high Vin SC converters. It includes level shifters, charge pumps and back-to-back switches. High Vin conversion is demonstrated in a 4:1 SC DCDC converter with an input voltage as high as 17V with a peak efficiency of 45 %, and a buckboost SC converter with an input voltage range starting from 2 up to 13V, which utilizes a total of 17 ratios and achieves a peak efficiency of 81.5 %. Furthermore a highly integrated micro power supply approach is introduced, which is connected directly to the 120/230 Vrms mains, with an output power of 3mW, resulting in a power density >390μW/mm², which exceeds prior art by a factor of 11.
Managing decentralized corporate energy systems is a challenging task for enterprises. However, the integration of energy objectives into business strategy creates difficulties resulting in inefficient decisions. To improve this, practice-proven methods such as the balanced scorecard and enterprise architecture management are transferred to the energy domain. The methods are evaluated based on a case study. Managing multi-dimensionality and high complexity are the main drivers for an effective and efficient energy management system. Both methods show a positive impact on managing decentralized corporate energy systems and are adaptable to the energy domain.
Decentralized energy systems are characterized by an ad hoc planing. The missing integration of energy objectives into business strategy creates difficulties resulting in inefficient energy architectures and decisions. Practice-proven methods such as balanced scorecard, enterprise architecture management and value network approach supports the transformation path towards an effective decentralized system. The methods are evaluated based on a case study. Managing multi-dimensionality, high complexity and multiple actors are the main drivers for an effective and efficient energy management system. The underlying basis to gain the positive impacts of these methods on decentralized corporate energy systems is digitization of energy data and processes.
This paper presents a control strategy for optimal utilization of photovoltaic (PV) generated power in conjunction with an Energy Storage System (ESS). The ESS is specifically designed to be retrofitted into existing PV systems in an end-user application. It can be attached in parallel to the PV system and connects to existing DC/AC inverters. In particular, the study covers the impact such a modification has on the output power of existing PV panels. A distinct degradation of PV output power was found due to the different power characteristics of PV panel and ESS. To overcome such degradation a novel feedback system is proposed. The feedback system continuously modifies the power characteristic of the ESS to match the PV panel and thus achieves optimal power utilization. Impact on PV and power point tracking performance is analyzed. Simulation of the proposed system is performed in MATLAB/Simulink. The results are found to be satisfactory.
A novel configuration of the dual active bridge (DAB) DC/DC converter is presented, enabling more efficient wide voltage range conversion at light loads. A third phase leg as well as a center tapped transformer are introduced to one side of the converter. This concept provides two different turn ratios, thus extending the zero voltage switching operation resulting in higher efficiency. A laboratory prototype was built converting an input voltage of 40V to an output voltage in the range of 350V to 650V. Measurements show a significant increase up to 20% in the efficiency for light-load operation.
Multilevel-cell (MLC) flash is commonly deployed in today’s high density NAND memories, but low latency and high reliability requirements make it barely used in automotive embedded flash applications. This paper presents a time domain voltage sensing scheme that applies a dynamic voltage ramp at the cells’ control gate (CG) in order to achieve fast and reliable sensing suitable for automotive applications.
Pre-clinical evaluation of advanced nerve guide conduits using a novel 3D in vitro testing model
(2018)
Autografts are the current gold standard for large peripheral nerve defects in clinics despite the frequently occurring side effects like donor site morbidity. Hollow nerve guidance conduits (NGC) are proposed alternatives to autografts, but failed to bridge gaps exceeding 3 cm in humans. Internal NGC guidance cues like microfibres are believed to enhance hollow NGCs by giving additional physical support for directed regeneration of Schwann cells and axons. In this study, we report a new 3D in vitro model that allows the evaluation of different intraluminal fibre scaffolds inside a complete NGC. The performance of electrospun polycaprolactone (PCL) microfibres inside 5 mm long polyethylene glycol (PEG) conduits were investigated in neuronal cell and dorsal root ganglion (DRG) cultures in vitro. Z-stack confocal microscopy revealed the aligned orientation of neuronal cells along the fibres throughout the whole NGC length and depth. The number of living cells in the centre of the scaffold was not significantly different to the tissue culture plastic (TCP) control. For ex vivo analysis, DRGs were placed on top of fibre-filled NGCs to simulate the proximal nerve stump. In 21 days of culture, Schwann cells and axons infiltrated the conduits along the microfibres with 2.2 ± 0.37 mm and 2.1 ± 0.33 mm, respectively. We conclude that this in vitro model can help define internal NGC scaffolds in the future by comparing different fibre materials, composites and dimensions in one setup prior to animal testing.
To deliver on a digital value proposition, companies must fundamentally re-architect. In other words, they must redesign their processes, systems, roles, data, and habits to allow them to iteratively create, enhance, an replace digital offerings. This briefing examines how Royal Philips is transforming its value proposition - and its entire company - to seize the opportunities presented by digital technologies.